SEC22B Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NB100-91277

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all SEC22B Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from amino acid region 2-50 of human SEC22B conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SEC22B Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: SEC22B Antibody - Azide and BSA Free [NB100-91277]

Immunohistochemistry-Paraffin: SEC22B Antibody - Azide and BSA Free [NB100-91277]

Immunohistochemistry-Paraffin: SEC22B Antibody [NB100-91277] - IHC on rat spinal cord using Rabbit antibody to SEC22B at a concentration of 30 ug/ml. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
Immunohistochemistry: SEC22B Antibody - Azide and BSA Free [NB100-91277]

Immunohistochemistry: SEC22B Antibody - Azide and BSA Free [NB100-91277]

Immunohistochemistry: SEC22B Antibody [NB100-91277] - IHC on rat spinal cord using Rabbit antibody to Vesicle-trafficking protein SEC22b (SEC22B, SEC22L1, ERS24): IgG at a concentration of 30 ug/ml. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
Immunohistochemistry: SEC22B Antibody - Azide and BSA Free [NB100-91277]

Immunohistochemistry: SEC22B Antibody - Azide and BSA Free [NB100-91277]

Immunohistochemistry: SEC22B Antibody [NB100-91277] - rat spinal cord using Rabbit antibody to Vesicle-trafficking protein SEC22b (SEC22B, SEC22L1, ERS24): IgG at a concentration of 30 ug/ml. Pre-absorption of the antibody with the immunizing peptide completely abolishes the immunostaining (not shown).
Immunohistochemistry: SEC22B Antibody - Azide and BSA Free [NB100-91277]

Immunohistochemistry: SEC22B Antibody - Azide and BSA Free [NB100-91277]

Immunohistochemistry: SEC22B Antibody [NB100-91277] - rat brain using Rabbit antibody to Vesicle-trafficking protein SEC22b (SEC22B, SEC22L1, ERS24): IgG at a concentration of 30 ug/ml. Pre-absorption of the antibody with the immunizing peptide completely abolishes the immunostaining (not shown).
Immunohistochemistry: SEC22B Antibody - Azide and BSA Free [NB100-91277]

Immunohistochemistry: SEC22B Antibody - Azide and BSA Free [NB100-91277]

Immunohistochemistry: SEC22B Antibody [NB100-91277] - rat spinal cord using Rabbit antibody to Vesicle-trafficking protein SEC22b (SEC22B, SEC22L1, ERS24): IgG at a concentration of 30 ug/ml. Pre-absorption of the antibody with the immunizing peptide completely abolishes the immunostaining (not shown).
Immunohistochemistry: SEC22B Antibody - Azide and BSA Free [NB100-91277]

Immunohistochemistry: SEC22B Antibody - Azide and BSA Free [NB100-91277]

Immunohistochemistry: SEC22B Antibody [NB100-91277] - rat spinal cord using Rabbit antibody to Vesicle-trafficking protein SEC22b (SEC22B, SEC22L1, ERS24): IgG at a concentration of 30 ug/ml. Pre-absorption of the antibody with the immunizing peptide completely abolishes the immunostaining (not shown).
Immunohistochemistry: SEC22B Antibody - Azide and BSA Free [NB100-91277]

Immunohistochemistry: SEC22B Antibody - Azide and BSA Free [NB100-91277]

Immunohistochemistry: SEC22B Antibody [NB100-91277] - IHC on rat brain using Rabbit antibody to SEC22B at a concentration of 30 ug/ml. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
Immunohistochemistry: SEC22B Antibody - Azide and BSA Free [NB100-91277]

Immunohistochemistry: SEC22B Antibody - Azide and BSA Free [NB100-91277]

Immunohistochemistry: SEC22B Antibody [NB100-91277] - IHC on rat spinal cord using Rabbit antibody to SEC22B at a concentration of 30 ug/ml. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

Applications for SEC22B Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

10-50 ug/ml

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

10-50 ug/ml

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Reconstitution

Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.

Formulation

Lyophilized from PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SEC22B

The protein encoded by this gene is a member of the SEC22 family of vesicle trafficking proteins. It seems to complex with SNARE and it is thought to play a role in the ER-Golgi protein trafficking. This protein has strong similarity to Mus musculus and Cricetulus griseus proteins. There is evidence for use of multiple polyadenylation sites for the transcript.

Alternate Names

ERS24, ERS-24sec22b, SEC22 vesicle trafficking protein homolog B (S. cerevisiae), SEC22 vesicle trafficking protein homolog B (S. cerevisiae) (gene/pseudogene), SEC22 vesicle trafficking protein-like 1, SEC22 vesicle trafficking protein-like 1 (S. cerevisiae), SEC22 vesicle-trafficking protein homolog B, SEC22 vesicle-trafficking protein-like 1, SEC22, vesicle trafficking protein (S. cerevisiae)-like 1, SEC22L1ER-Golgi SNARE of 24 kDa, vesicle-trafficking protein SEC22b

Entrez Gene IDs

9554 (Human); 20333 (Mouse); 310710 (Rat)

Gene Symbol

SEC22B

UniProt

O75396 (Human)

Additional SEC22B Products

Product Documents for SEC22B Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SEC22B Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SEC22B Antibody - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for SEC22B Antibody - Azide and BSA Free

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  • Q: Could you tell me to which part of the protein the antibody is directed to? I would like to use it for co-immunoprecipitation studies and I want to know if the antibody would intefere with the binding of sec22b to other proteins.

    A: Our SEC22B antibody with catalogue number NB100-91277 was raised to a synthetic peptide derived from amino acid region 2-50 of the human protein. According to the Uniprot listing this region is cytoplasmic: Uniprot site

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