SEC61A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31267

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%), Rhesus Macaque (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SEC61A Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SEC61A. The exact sequence is proprietary.

Localization

Endoplasmic reticulum membrane

Specificity

This antibody made against SEC61A1 but the immunogen shows 78% sequence identity with human SEC61A2. Reactivity with SEC61A2 has not been determined experimentally.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SEC61A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SEC61A Antibody [NBP1-31267]

Immunocytochemistry/ Immunofluorescence: SEC61A Antibody [NBP1-31267]

Immunocytochemistry/Immunofluorescence: SEC61A Antibody [NBP1-31267] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: SEC61A Antibody [NBP1-31267]

Immunohistochemistry-Paraffin: SEC61A Antibody [NBP1-31267]

Immunohistochemistry-Paraffin: SEC61A Antibody [NBP1-31267] - Cal27 xenograft, using SEC61A1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
SEC61A Antibody

Western Blot: SEC61A Antibody [NBP1-31267] -

Western Blot: SEC61A Antibody [NBP1-31267] - SEC61A antibody detects SEC61A protein by Western blot analysis. K562 whole cell extracts and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEC61A antibody (NBP1-31267) diluted at 1:1000.

Applications for SEC61A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SEC61A

SEC61A belongs to the SECY/SEC61 alpha family. It has similarity to a mouse protein which suggests a role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum.The SEC61 complex is responsible for translocation of proteins across the rough endoplasmic reticulum membrane. SEC61 is composed of three subunits: alpha, beta, and gamma; that associate to form a heterotrimer. Multiple heterotrimers associate forming the ER translocon. SEC61 alpha is a polytopic protein that has ten membrane-spanning loops. SEC61 has been found to be tightly associated with membrane bound ribosomes, either directly or through adapter proteins, and is required for assembly of membrane and secretory proteins.

Alternate Names

HSEC61, protein transport protein SEC61 alpha subunit, protein transport protein Sec61 subunit alpha, protein transport protein Sec61 subunit alpha isoform 1, SEC61, Sec61 alpha 1 subunit (S. cerevisiae), Sec61 alpha-1, sec61 homolog, SEC61A

Gene Symbol

SEC61A1

Additional SEC61A Products

Product Documents for SEC61A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SEC61A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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