Secretagogin Antibody

Novus Biologicals | Catalog # NBP2-20286

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Secretagogin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Secretagogin. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (84%), Xenopus tropicalis (84%).

Localization

Cytoplasm; Secreted (By similarity); Cytoplasmic vesicle; secretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side (By similarity)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Secretagogin Antibody

Western Blot: Secretagogin Antibody [NBP2-20286]

Western Blot: Secretagogin Antibody [NBP2-20286]

Western Blot: Secretagogin Antibody [NBP2-20286] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Secretagogin antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: Secretagogin Antibody [NBP2-20286]

Immunocytochemistry/ Immunofluorescence: Secretagogin Antibody [NBP2-20286]

Immunocytochemistry/Immunofluorescence: Secretagogin Antibody [NBP2-20286] - Secretagogin antibody detects Secretagogin protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Secretagogin stained by Secretagogin antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286]

Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286]

Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286] - Secretagogin antibody detects Secretagogin protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Secretagogin protein stained by Secretagogin antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
Western Blot: Secretagogin Antibody [NBP2-20286]

Western Blot: Secretagogin Antibody [NBP2-20286]

Western Blot: Secretagogin Antibody [NBP2-20286] - Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Secretagogin Antibody [NBP2-20286]

Immunohistochemistry-Paraffin: Secretagogin Antibody [NBP2-20286]

Immunohistochemistry-Paraffin: Secretagogin Antibody [NBP2-20286] - Human colon carcinoma, using SCGN antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286]

Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286]

Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286] - Secretagogin antibody detects Secretagogin protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: Secretagogin protein stained by Secretagogin antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286]

Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286]

Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286] - Secretagogin antibody detects Secretagogin Protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: Secretagogin stained by Secretagogin antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI.

Applications for Secretagogin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Secretagogin

The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation. [provided by RefSeq]

Alternate Names

CALBL, SCGN, SECRET, SEGN, Setagin

Gene Symbol

SCGN

UniProt

O76038 (Human)

Additional Secretagogin Products

Product Documents for Secretagogin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Secretagogin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Secretagogin Antibody

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Protocols

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FAQs

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