Secretogranin 3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89825

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SCG3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LDGTPLTAEDIVHKIAARIYEENDRAVFDKIVSKLLNLGLITESQAHTLEDEVAEVLQKLISKEANNYEEDPNKPTSWTENQAGKIPEKVTPMAAIQDGLAKGE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Secretogranin 3 Antibody - BSA Free

Secretogranin 3 Antibody - BSA Free Immunohistochemistry-Paraffin: Secretogranin 3 Antibody - BSA Free [NBP1-89825]

Immunohistochemistry-Paraffin: Secretogranin 3 Antibody - BSA Free [NBP1-89825]

Analysis in human cerebral cortex and cervix, uterine tissues using NBP1-89825 antibody. Corresponding SCG3 RNA-seq data are presented for the same tissues.
Secretogranin 3 Antibody - BSA Free Immunohistochemistry-Paraffin: Secretogranin 3 Antibody - BSA Free [NBP1-89825]

Immunohistochemistry-Paraffin: Secretogranin 3 Antibody - BSA Free [NBP1-89825]

Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons and moderate cytoplasmic positivity in neuropil.
Secretogranin 3 Antibody - BSA Free Immunohistochemistry-Paraffin: Secretogranin 3 Antibody - BSA Free [NBP1-89825]

Immunohistochemistry-Paraffin: Secretogranin 3 Antibody - BSA Free [NBP1-89825]

Staining of human cervix, uterine shows no positivity in squamous epithelial cells as expected.
Secretogranin 3 Antibody - BSA Free Immunohistochemistry-Paraffin: Secretogranin 3 Antibody - BSA Free [NBP1-89825]

Immunohistochemistry-Paraffin: Secretogranin 3 Antibody - BSA Free [NBP1-89825]

Staining of human liver shows no positivity in hepatocytes as expected.
Secretogranin 3 Antibody - BSA Free Immunohistochemistry-Paraffin: Secretogranin 3 Antibody - BSA Free [NBP1-89825]

Immunohistochemistry-Paraffin: Secretogranin 3 Antibody - BSA Free [NBP1-89825]

Staining of human pancreas shows strong cytoplasmic positivity in endocrine glandular cells.
Secretogranin 3 Antibody - BSA Free Western Blot: Secretogranin 3 Antibody - BSA Free [NBP1-89825]

Western Blot: Secretogranin 3 Antibody - BSA Free [NBP1-89825]

Analysis in human cell line SH-SY5Y.

Applications for Secretogranin 3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SCG3

Secretogranin 3 is encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Granins may serve as precursors for biologically active peptides. Some granins have been shown to function as helper proteins in sorting and proteolytic processing of prohormones; however, the function of this protein is unknown. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Long Name

Secretogranin III

Alternate Names

Secretogranin III, Secretogranin-3, SGIII

Gene Symbol

SCG3

Additional SCG3 Products

Product Documents for Secretogranin 3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Secretogranin 3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Secretogranin 3 Antibody - BSA Free

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Protocols

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