Semaphorin 6A Antibody

Novus Biologicals | Catalog # NBP3-23387

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Chicken, Xenopus

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Semaphorin 6A synthetic peptide (coupled to carrier protein) corresponding to amino acids in the cytoplasmic region of human Sema-6A. The sequence used has high homology to similar regions in rat and mouse Sema-6A, and has low homology to other semaphorin family members. [UniProt# Q9H2E6]

Specificity

The antibody detects a 120kDa protein corresponding to the apparent molecular mass ofSemaphorin 6A on SDS-PAGE immunoblots of Sema-6A transfected COS7 cells, human colorectal cancer cells, and rat PC12 cells. In addition, shRNA knockdown of Sema-6A expression significantly reduces detection of Sema-6A using anti-Sema-6A (a.a. 772-787) antibody.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Applications for Semaphorin 6A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:250

Immunoprecipitation

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Semaphorin 6A

Can act as repulsive axon guidance cues. May play a role in channeling sympathetic axons into the sympathetic chains and controlling the temporal sequence of sympathetic target innervation.

Alternate Names

Sema6A

Gene Symbol

SEMA6A

Additional Semaphorin 6A Products

Product Documents for Semaphorin 6A Antibody

Certificate of Analysis

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Product Specific Notices for Semaphorin 6A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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