SENP3 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-56590
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Key Product Details
Species Reactivity
Human, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Synthetic peptides corresponding to SENP3(SUMO1/sentrin/SMT3 specific peptidase 3) The peptide sequence was selected from the N terminal of SENP3.
Peptide sequence PPPKPRLKSGGGFGPDPGSGTTVPARRLPVPRPSFDASASEEEEEEEEEE. The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for SENP3 Antibody - BSA Free
Western Blot: SENP3 Antibody [NBP1-56590]
Western Blot: SENP3 Antibody [NBP1-56590] - Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1 : 1562500 Positive control: 721_B cell lysatesENP3 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.Immunohistochemistry: SENP3 Antibody [NBP1-56590]
Immunohistochemistry: SENP3 Antibody [NBP1-56590] - HeLa cells using Immunohistochemistry. SENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa.Western Blot: SENP3 Antibody [NBP1-56590]
Western Blot: SENP3 Antibody [NBP1-56590] - Hela cell lysate, Antibody Titration: 0.2-1 ug/ml.Western Blot: SENP3 Antibody [NBP1-56590]
Western Blot: SENP3 Antibody [NBP1-56590] - Sample Type: 1. Rat Liver cells (200ug) 2. Rat Brain cells (200ug) 3. Rat Testis cells (200ug) 4. Rat Kidney cells (200ug) Primary Dilution: 1:1000 Secondary Antibody: alkaline phosphatase-conjugated anti-rabbit Secondary Dilution: 1:1000 Image Submitted by: Andreia Carvalho IBMC-OBF, Portugal.Western Blot: SENP3 Antibody [NBP1-56590]
Western Blot: SENP3 Antibody [NBP1-56590] - 1. HeLa cell extract (200ug) 2. HeLa cell extract (200ug) incubated with HA-SUMO2-VME *HA-S2-VME-SENP3 Primary Dilution: 1:1000 Secondary Antibody: alkaline phosphatase-conjugated anti-rabbit Secondary Dilution: 1:1000.Western Blot: SENP3 Antibody [NBP1-56590]
Western Blot: SENP3 Antibody [NBP1-56590] - Rat Liver cells (200ug) 2. Rat Brain cells (200ug) 3. Rat Testis cells (200ug) 4. Rat Kidney cells (200ug) at 1:1000.Western Blot: SENP3 Antibody [NBP1-56590]
Western Blot: SENP3 Antibody [NBP1-56590] - 1. HeLa cell extract (200ug) Primary Dilution: 1 : 1000 Secondary Antibody: alkaline phosphatase-conjugated anti-rabbit Secondary Dilution: 1 : 1000.Applications for SENP3 Antibody - BSA Free
Application
Recommended Usage
Western Blot
1.0 ug/ml
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SENP3
Alternate Names
DKFZP586K0919, DKFZp762A152, EC 3.4.22, EC 3.4.22.-, sentrin/SUMO-specific protease 3, Sentrin/SUMO-specific protease SENP3, sentrin-specific protease 3, SMT3IP1, SSP3DKFZp586K0919, SUMO1/sentrin/SMT3 specific peptidase 3, SUMO1/sentrin/SMT3 specific protease 3, SUMO-1-specific protease 3, SUSP3
Gene Symbol
SENP3
Additional SENP3 Products
Product Documents for SENP3 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SENP3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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