Serpin A3/alpha 1-Antichymotrypsin Antibody (CL10697) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-44021
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL10697
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of P01011, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images
![Serpin A3/alpha 1-Antichymotrypsin Antibody (CL10697) - Azide and BSA Free Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody [NBP3-44021]](https://resources.rndsystems.com/images/products/nbp3-44021_mouse-serpin-a3-alpha-1-antichymotrypsin-mab-cl10697-28220251219546.jpg "Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody [NBP3-44021]")
![Serpin A3/alpha 1-Antichymotrypsin Antibody (CL10697) - Azide and BSA Free Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody [NBP3-44021]](https://resources.rndsystems.com/images/products/nbp3-44021_mouse-serpin-a3-alpha-1-antichymotrypsin-mab-cl10697-282202512384240.jpg "Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody [NBP3-44021]")
Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody [NBP3-44021]
Staining of human cerebral cortex shows cytoplasmic and nuclear positivity in a subset of glial cells.![Serpin A3/alpha 1-Antichymotrypsin Antibody (CL10697) - Azide and BSA Free Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody [NBP3-44021]](https://resources.rndsystems.com/images/products/nbp3-44021_mouse-serpin-a3-alpha-1-antichymotrypsin-mab-cl10697-282202513135979.jpg "Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody [NBP3-44021]")
Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody [NBP3-44021]
Staining of human skeletal muscle shows no positivity in myocytes as expected.![Serpin A3/alpha 1-Antichymotrypsin Antibody (CL10697) - Azide and BSA Free Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody [NBP3-44021]](https://resources.rndsystems.com/images/products/nbp3-44021_mouse-serpin-a3-alpha-1-antichymotrypsin-mab-cl10697-28220251344287.jpg "Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody [NBP3-44021]")
Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody [NBP3-44021]
Staining of human liver shows strong cytoplasmic positivity in hepatocytes.![Serpin A3/alpha 1-Antichymotrypsin Antibody (CL10697) - Azide and BSA Free Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody [NBP3-44021]](https://resources.rndsystems.com/images/products/nbp3-44021_mouse-serpin-a3-alpha-1-antichymotrypsin-mab-cl10697-28220251339170.jpg "Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody [NBP3-44021]")
Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody [NBP3-44021]
Staining of human uterine cervix shows moderate cytoplasmic positivity in squamous epithelial cells.Applications
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:2500 - 1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: Serpin A3/alpha 1-Antichymotrypsin
Alternate Names
alpha 1-Antichymotrypsin
Gene Symbol
SERPINA3
Additional Serpin A3/alpha 1-Antichymotrypsin Products
Product Documents
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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