SerpinB2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33188

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Serpin B2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human SerpinB2. The exact sequence is proprietary.

Reactivity Notes

Rabbit (83%), Sheep (81%), Bovine (82%).

Localization

Cytoplasm, Secreted, extracellular space

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SerpinB2 Antibody - BSA Free

Western Blot: SerpinB2 Antibody [NBP1-33188]

Western Blot: SerpinB2 Antibody [NBP1-33188]

Western Blot: SerpinB2 Antibody [NBP1-33188] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAI2 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: SerpinB2 Antibody [NBP1-33188]

Immunocytochemistry/ Immunofluorescence: SerpinB2 Antibody [NBP1-33188]

Immunocytochemistry/Immunofluorescence: SerpinB2 Antibody [NBP1-33188] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PAI2 protein stained by PAI2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: SerpinB2 Antibody [NBP1-33188]

Immunohistochemistry-Paraffin: SerpinB2 Antibody [NBP1-33188]

Immunohistochemistry-Paraffin: SerpinB2 Antibody [NBP1-33188] - NCIN87 xenograft, using PAI-2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunoprecipitation: SerpinB2 Antibody [NBP1-33188]

Immunoprecipitation: SerpinB2 Antibody [NBP1-33188]

Immunoprecipitation: SerpinB2 Antibody [NBP1-33188] - Immunoprecipitation of PAI2 protein from U87-MG whole cell extracts using 5 ug of PAI2 antibody. Western blot analysis was performed using PAI2 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
SerpinB2 Antibody

Western Blot: SerpinB2 Antibody [NBP1-33188] -

Western Blot: SerpinB2 Antibody [NBP1-33188] -Sample (30 μg of whole cell lysate)A: U87-MG10% SDS PAGEdiluted at 1:10000The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SerpinB2 Antibody

Western Blot: SerpinB2 Antibody [NBP1-33188] -

Western Blot: SerpinB2 Antibody [NBP1-33188] -Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PAI2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for SerpinB2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Serpin B2

Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1

Alternate Names

PAI2, PLANH2, Urokinase Inhibitor

Gene Symbol

SERPINB2

Additional Serpin B2 Products

Product Documents for SerpinB2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SerpinB2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SerpinB2 Antibody - BSA Free

Customer Reviews for SerpinB2 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review SerpinB2 Antibody - BSA Free and earn rewards!

Have you used SerpinB2 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies