SETD1A Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-49281
![Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]](https://resources.rndsystems.com/images/products/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP2-49281-img0005.jpg "Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]")
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Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: SQFRSSDANYPAYYESWNRYQRHTSYPPRRATREEPPGAPFAENTAERFPPSYTSYLPPEPSRPTDQDYRPPASEA
Reactivity Notes
Mouse (88%), Rat (89%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit SETD1A Antibody - BSA Free (NBP2-49281) is a polyclonal antibody validated for use in IHC. Anti-SETD1A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for SETD1A Antibody - BSA Free
![Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]](https://resources.rndsystems.com/images/products/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP2-49281-img0006.jpg "Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]")
Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]
Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281] - Staining of human cerebral cortex, kidney, pancreas and testis using Anti-SETD1A antibody NBP2-49281 (A) shows similar protein distribution across tissues to independent antibody NBP1-81513 (B).![Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]](https://resources.rndsystems.com/images/products/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP2-49281-img0012.jpg "Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]")
Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]
Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281] - Staining of human Pancreas shows moderate nuclear positivity in exocrine glandular cells.![Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]](https://resources.rndsystems.com/images/products/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP2-49281-img0003.jpg "Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]")
Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]
Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281] - Staining of human pancreas shows low expression as expected.![Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]](https://resources.rndsystems.com/images/products/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP2-49281-img0004.jpg "Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]")
Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]
Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281] - Staining of human testis shows high expression.![Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]](https://resources.rndsystems.com/images/products/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP2-49281-img0007.jpg "Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]")
Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]
Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281] - Staining of human cerebral cortex.![Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]](https://resources.rndsystems.com/images/products/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP2-49281-img0008.jpg "Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]")
Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]
Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281] - Staining of human kidney.![Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]](https://resources.rndsystems.com/images/products/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP2-49281-img0009.jpg "Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]")
Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]
Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281] - Staining of human Cerebral cortex shows moderate nuclear positivity in neuronal and glial cells.![Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]](https://resources.rndsystems.com/images/products/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP2-49281-img0010.jpg "Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]")
Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]
Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281] - Staining of human cerebral cortex, kidney, pancreas and testis using Anti-SETD1A antibody NBP2-49281 (A) shows similar protein distribution across tissues to independent antibody NBP1-81513 (B).![Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]](https://resources.rndsystems.com/images/products/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP2-49281-img0011.jpg "Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]")
Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]
Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281] - Staining of human Kidney shows moderate nuclear positivity in cells in tubules and glomeruli.![Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]](https://resources.rndsystems.com/images/products/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP2-49281-img0013.jpg "Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]")
Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281]
Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281] - Staining of human Testis shows strong nuclear positivity in cells in seminiferous ducts.Applications for SETD1A Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SETD1A
Alternate Names
histone-lysine N-methyltransferase SETD1A, hSET1A, KIAA0339EC 2.1.1.43, KMT2FSET1, Lysine N-methyltransferase 2F, SET domain containing 1A, SET domain-containing protein 1A, Set1, Set1/Ash2 histone methyltransferase complex subunit SET1, SET1A
Gene Symbol
SETD1A
Additional SETD1A Products
Product Documents for SETD1A Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SETD1A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for SETD1A Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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