SETDB1 Antibody (HL3260)

Novus Biologicals | Catalog # NBP3-48557

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL3260
View all SETDB1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human SETDB1.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SETDB1 Antibody (HL3260)

SETDB1 Antibody (HL3260)

Western Blot: SETDB1 Antibody (HL3260) [NBP3-48557] -

Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL3260] (NBP3-48557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SETDB1 Antibody (HL3260)

Immunocytochemistry/ Immunofluorescence: SETDB1 Antibody (HL3260) [NBP3-48557] -

SETDB1 antibody [HL3260] detects SETDB1 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SETDB1 stained by SETDB1 antibody [HL3260] (NBP3-48557) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
SETDB1 Antibody (HL3260)

Western Blot: SETDB1 Antibody (HL3260) [NBP3-48557] -

HeLa whole cell and nuclear extract (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL3260] (NBP3-48557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for SETDB1 Antibody (HL3260)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SETDB1

The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila Trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 039;Enhancer of zeste 039 proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities. [supplied by OMIM]

Alternate Names

ERG-associated protein with SET domain, ESETEC 2.1.1.43, H3-K9-HMTase 4, Histone H3-K9 methyltransferase 4, histone-lysine N-methyltransferase SETDB1, histone-lysine N-methyltransferase, H3lysine-9 specific 4, KG1T, KIAA0067H3-K9-HMTase4, KMT1EERG-associated protein with a SET domain, ESET, Lysine N-methyltransferase 1E, SET domain bifurcated 1, SET domain, bifurcated 1

Gene Symbol

SETDB1

Additional SETDB1 Products

Product Documents for SETDB1 Antibody (HL3260)

Certificate of Analysis

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Product Specific Notices for SETDB1 Antibody (HL3260)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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