SETDB1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77990

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SETDB1 Primary Antibodies »

Product Specifications

Immunogen

This affinity purified SETDB1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1050-1075 of human SETDB1. (Uniprot: Q15047)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for SETDB1 Antibody - BSA Free

Western Blot: SETDB1 Antibody [NBP1-77990]

Western Blot: SETDB1 Antibody [NBP1-77990]

Western Blot: SETDB1 Antibody [NBP1-77990] - detect human ESET present in a 293 whole cell lysate. ~30ug of lysate was loaded per lane for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a single band of ~170 kDa is detected. Peptide competition (not shown) blocks staining of this band. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min.

Applications for SETDB1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:10000-1:50000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000-1:2500
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blotting. Western blotting shows reactivity specific for human ESET detecting a band at approximately 170 kDa. Specific band detection by western blot is blocked by peptide competition by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane. Reactivity in other immunoassays is unknown.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: SETDB1

The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities. ESET functions as a histone methyltransferase by methylation of Lys-9 of histone H3. H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. ESET shows a nuclear localization and is associated with non-pericentromeric regions of chromatin, and is excluded from nucleoli and islands of condensed chromatin. Although ESET is a widely expressed protein, it is highly expressed in the testis.

Alternate Names

ERG-associated protein with SET domain, ESETEC 2.1.1.43, H3-K9-HMTase 4, Histone H3-K9 methyltransferase 4, histone-lysine N-methyltransferase SETDB1, histone-lysine N-methyltransferase, H3lysine-9 specific 4, KG1T, KIAA0067H3-K9-HMTase4, KMT1EERG-associated protein with a SET domain, ESET, Lysine N-methyltransferase 1E, SET domain bifurcated 1, SET domain, bifurcated 1

Entrez Gene IDs

9869 (Human)

Gene Symbol

SETDB1

UniProt

Q15047 (Human)

Additional SETDB1 Products

Product Documents for SETDB1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SETDB1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SETDB1 Antibody - BSA Free

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Protocols

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