SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-44229
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL9101
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was generated using a synthetic peptide of Q13285, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images
![SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101) - Azide and BSA Free Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP3-44229]](https://resources.rndsystems.com/images/products/nbp3-44229_mouse-sf-1-nr5a1-steroidogenic-factor-1-mab-cl9101-282202512145365.jpg "Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP3-44229]")
Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP3-44229]
Staining of human adrenal gland shows moderate nuclear positivity in glandular cells.![SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101) - Azide and BSA Free Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP3-44229]](https://resources.rndsystems.com/images/products/nbp3-44229_mouse-sf-1-nr5a1-steroidogenic-factor-1-mab-cl9101-28220251294020.jpg "Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP3-44229]")
Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP3-44229]
Staining of human breast shows weak to moderate nuclear positivity in glandular cells.![SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101) - Azide and BSA Free Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP3-44229]](https://resources.rndsystems.com/images/products/nbp3-44229_mouse-sf-1-nr5a1-steroidogenic-factor-1-mab-cl9101-282202513121853.jpg "Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP3-44229]")
Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP3-44229]
Staining of human ovary shows moderate positivity in stromal cells.![SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101) - Azide and BSA Free Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP3-44229]](https://resources.rndsystems.com/images/products/nbp3-44229_mouse-sf-1-nr5a1-steroidogenic-factor-1-mab-cl9101-282202512495890.jpg "Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP3-44229]")
Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP3-44229]
Staining of human liver shows no positivity in hepatocytes as expected.![SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101) - Azide and BSA Free Western Blot: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP3-44229]](https://resources.rndsystems.com/images/products/nbp3-44229_mouse-sf-1-nr5a1-steroidogenic-factor-1-mab-cl9101-282202513314652.jpg "Western Blot: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP3-44229]")
Western Blot: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP3-44229]
Analysis in human cell line HEL.![SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101) - Azide and BSA Free Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP3-44229]](https://resources.rndsystems.com/images/products/nbp3-44229_mouse-sf-1-nr5a1-steroidogenic-factor-1-mab-cl9101-282202513245491.jpg "Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP3-44229]")
Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP3-44229]
Staining of human testis shows weak to moderate nuclear positivity in Leydig cells and cells in seminiferous ducts.![SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP3-44229]](https://resources.rndsystems.com/images/products/nbp3-44229_mouse-sf-1-nr5a1-steroidogenic-factor-1-mab-cl9101-28220251339196.jpg "Immunocytochemistry/ Immunofluorescence: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP3-44229]")
Immunocytochemistry/ Immunofluorescence: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP3-44229]
Staining of U-2 OS cells using the Anti-SF-1/NR5A1/Steroidogenic Factor 1 monoclonal antibody).Applications
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1 ug/ml
Application Notes
For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: SF-1/NR5A1
Long Name
Steroidogenic Factor 1
Alternate Names
AD4BP, FTZF1, NR5A1, SF1
Gene Symbol
NR5A1
Additional SF-1/NR5A1 Products
Product Documents
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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