sFRP-4 Antibody

Name: sFRP-4 Antibody
Brand: Novus Biologicals
SKU: NBP1-36985
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-36985

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Citations (1)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

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Product Specifications

Immunogen

Peptide with sequence TNSSCQCPHILPHQD corresponding to internal region according to NP_003005.2.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for sFRP-4 Antibody

Western Blot: sFRP-4 Antibody [NBP1-36985]

Western Blot: sFRP-4 Antibody [NBP1-36985] - Western blot analysis of SFRP4 in Human Uterus lysate (35ug protein in RIPA buffer) using NBP1-36985 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-36985]

Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-36985] - Human Uterus shows granular staining in the apical cytoplasm of the endometrial gland.

Applications for sFRP-4 Antibody

Application

Recommended Usage

Immunohistochemistry

2 - 4 ug/ml

Immunohistochemistry-Paraffin

2 - 4 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml

Application Notes

WB: Approx. 40 kDa band observed in human uterus lysates (calculated MW of 39.8 kDa band according to NP_003005.2). IHC-P: Human uterus shows granular staining in the apical cytoplasm of the endometrial gland.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: sFRP-4

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression.

Long Name

Secreted Frizzled Related Protein 4

Alternate Names

DDC-4, FRP-AP, frpHE, FrzB2, sFRP4

Entrez Gene IDs

20379 (Mouse); 89803 (Rat)

Gene Symbol

SFRP4

UniProt

NP_003005.2 (Human)

Additional sFRP-4 Products

Product Documents for sFRP-4 Antibody

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Certificate of Analysis

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Product Specific Notices for sFRP-4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Wnt Family

Wnt Inhibitors

Citations for sFRP-4 Antibody

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