sFRP-4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81866

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (97%), Rat (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all sFRP-4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RHMPWNITRMPNHLHHSTQENAILAIEQYEELVDVNCSAVLRFFLCAMYAPICTLEFLHDPIKPCKSVCQRARDDCEPLMKMYNHSWPESLACDELPVYDRGVCISPEAIVTDLPEDVKWIDITPDMMVQERPLDVDCKR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for sFRP-4 Antibody - BSA Free

Western Blot: sFRP-4 Antibody [NBP1-81866]

Western Blot: sFRP-4 Antibody [NBP1-81866]

Western Blot: sFRP-4 Antibody [NBP1-81866] - Analysis in control (vector only transfected HEK293T lysate) and sFRP4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-81866]

Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-81866]

Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-81866] - Staining of human placenta shows no positivity in trophoblastic cells as expected.
Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-81866]

Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-81866]

Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-81866] - Staining of human endometrium shows moderate secreted positivity in cells in endometrial stroma.
Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-81866]

Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-81866]

Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-81866] - Staining of human kidney shows no positivity in cells in tubules as expected.
Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-81866]

Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-81866]

Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-81866] - Staining of human liver shows no positivity in hepatocytes as expected.
sFRP-4 Antibody Immunohistochemistry-Paraffin: sFRP-4 Antibody Antibody [NBP1-81866]

Immunohistochemistry-Paraffin: sFRP-4 Antibody Antibody [NBP1-81866]

Staining of human endometrium, kidney, liver and placenta using NBP1-81866 (A) shows similar protein distribution across tissues to independent antibody NBP2-38632 (B).

Applications for sFRP-4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-P reported in scientific literature (PMID: 25232495). For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: sFRP-4

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression.

Long Name

Secreted Frizzled Related Protein 4

Alternate Names

DDC-4, FRP-AP, frpHE, FrzB2, sFRP4

Gene Symbol

SFRP4

Additional sFRP-4 Products

Product Documents for sFRP-4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for sFRP-4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Wnt Family
Wnt Inhibitors

Citations for sFRP-4 Antibody - BSA Free

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Protocols

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FAQs

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