![Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632]](https://beta-resources.rndsystems.com/images/products/sFRP-4-Antibody-Immunohistochemistry-Paraffin-NBP2-38632-img0017.jpg "Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632]")
Loading...
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human
Predicted:
Mouse (92%), Rat (92%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: PTLATYLSKNYSYVIHAKIKAVQRSGCNEVTTVVDVKEIFKSSSPIPRTQVPLITNSSCQCPHILPHQDVLIMCYEWRSRMMLLENCLVEKWRDQLSKRSIQWEERLQEQRRTVQDKKKTAGRTSRSNPPKPKGKPPAPKPASPK
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit sFRP-4 Antibody - BSA Free (NBP2-38632) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for sFRP-4 Antibody - BSA Free
![Western Blot: sFRP-4 Antibody [NBP2-38632]](https://beta-resources.rndsystems.com/images/products/sFRP-4-Antibody-Western-Blot-NBP2-38632-img0002.jpg "Western Blot: sFRP-4 Antibody [NBP2-38632]")
Western Blot: sFRP-4 Antibody [NBP2-38632]
Western Blot: sFRP-4 Antibody [NBP2-38632] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma![Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632]](https://beta-resources.rndsystems.com/images/products/sFRP-4-Antibody-Immunohistochemistry-Paraffin-NBP2-38632-img0012.jpg "Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632]")
Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632]
Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632] - Staining of human endometrium shows moderate secreted positivity in cells in endometrial stroma.![Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632]](https://beta-resources.rndsystems.com/images/products/sFRP-4-Antibody-Immunohistochemistry-Paraffin-NBP2-38632-img0013.jpg "Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632]")
Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632]
Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632] - Staining of human kidney shows no positivity in cells in tubules as expected.![Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632]](https://beta-resources.rndsystems.com/images/products/sFRP-4-Antibody-Immunohistochemistry-Paraffin-NBP2-38632-img0014.jpg "Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632]")
Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632]
Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632] - Staining of human liver shows no positivity in hepatocytes as expected.![Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632]](https://beta-resources.rndsystems.com/images/products/sFRP-4-Antibody-Immunohistochemistry-Paraffin-NBP2-38632-img0015.jpg "Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632]")
Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632]
Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632] - Staining of human placenta shows no positivity in trophoblastic cells as expected.Applications for sFRP-4 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Reviewed Applications
Read 1 review rated 4 using NBP2-38632 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: sFRP-4
Long Name
Secreted Frizzled Related Protein 4
Alternate Names
DDC-4, FRP-AP, frpHE, FrzB2, sFRP4
Gene Symbol
SFRP4
Additional sFRP-4 Products
Product Documents for sFRP-4 Antibody - BSA Free
Product Specific Notices for sFRP-4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for sFRP-4 Antibody - BSA Free (1)
4 out of 5
1 Customer Rating
Have you used sFRP-4 Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Customer Images
Showing
1
-
1 of
1 review
Showing All
Filter By:
-
Application: Western BlotSample Tested: Whole cell lysateSpecies: HumanVerified Customer | Posted 09/30/2019These have been attached. The protein content of all samples were quantified prior to loading, and standardized at concentrations of 40ug.
There are no reviews that match your criteria.
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...