SGK1 Antibody

Novus Biologicals | Catalog # NBP2-19010

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Baboon (100%), Bovine (95%), Canine (95%), Chimpanzee (100%), Equine (95%), Monkey (100%), Mouse (90%), Opossum (100%), Porcine (95%), Rat (90%), Rhesus Macaque (100%), Zebrafish (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SGK1 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 360-410 from human SGK1 was used as the immunogen for this antibody. NP_005618.2.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: 100% homologous in new world monkey, macaque, rhesus monkey, opossum, gibbon, orangutan, and chimpanzee; 95% in panda, carolina anole, cow, dog, chinese hamster, zebrafish, horse, mole rat, elephant, platypus, and pig; 90% in mouse, rat, zebra finch, frog, cichlid fish, rabbit, guinea pig, chicken, and wild turkey; 85% in salmon.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SGK1 Antibody

Western Blot: SGK1 Antibody [NBP2-19010]

Western Blot: SGK1 Antibody [NBP2-19010]

Western Blot: SGK1 Antibody [NBP2-19010] - Analysis of SGK1 in human lung lysate in the 1) absence and 2) presence of immunizing peptide, 3) human kidney lysate probed with 1 ug/ml of SGK1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this western blot.
Immunohistochemistry-Paraffin: SGK1 Antibody [NBP2-19010]

Immunohistochemistry-Paraffin: SGK1 Antibody [NBP2-19010]

Immunohistochemistry-Paraffin: SGK1 Antibody [NBP2-19010] - analysis of SGK1 in human lung probed with SGK1 antibody at 5 ug/ml.

Applications for SGK1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-3 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SGK1

SGK1, an acronym for serum and glucocorticoid regulated kinase, appears to be important for intrauterine embryo implantation and pregnancy maintenance along with a variety of other pathways such as neuronal excitation, renal sodium ion secretion, apoptosis and T-cell signaling. SGK1 activity and stability appears to have an inverse correlation with ubiquitinylation pathways. SGK1 has an interaction with Notch through Fbw as the absence of SGK1 promotes Notch protein stability and transcriptional activity. SGK1 association with Tcell signaling is also indicated as Notch and Fbw7 mutations are found in a majority of T-ALL cases. SGK1 antibodies can be useful for further analysis of the relative importance of this kinase in numerous diverse cell processes.

Long Name

Serum/Glucocorticoid Regulated Kinase 1

Alternate Names

SGK

Gene Symbol

SGK1

Additional SGK1 Products

Product Documents for SGK1 Antibody

Certificate of Analysis

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Product Specific Notices for SGK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

mTOR Signaling Pathway mTOR Signaling Pathway Thumbnail