SGLT1/SLC5A1 Antibody - Azide Free

Novus Biologicals | Catalog # NBP2-20338

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Predicted:

Canine (90%), Rabbit (90%), Sheep (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/ICC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human SGLT1/SLC5A1. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (80%), Cat (89%), Pig (88%), Chicken (82%), Bovine (89%).

Localization

Membrane; Multi-pass membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SGLT1/SLC5A1 Antibody - Azide Free

Immunocytochemistry/ Immunofluorescence: SGLT1/SLC5A1 Antibody [NBP2-20338]

Immunocytochemistry/ Immunofluorescence: SGLT1/SLC5A1 Antibody [NBP2-20338]

Immunocytochemistry/Immunofluorescence: SGLT1/SLC5A1 Antibody [NBP2-20338] - Analysis of methanol-fixed A431, using SGLT1antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: SGLT1/SLC5A1 Antibody [NBP2-20338]

Immunohistochemistry-Paraffin: SGLT1/SLC5A1 Antibody [NBP2-20338]

Immunohistochemistry-Paraffin: SGLT1/SLC5A1 Antibody [NBP2-20338] - Human lung fibrose tissue, using SGLT1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
SGLT1/SLC5A1 Antibody - Azide Free

Western Blot: SGLT1/SLC5A1 Antibody [NBP2-20338] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SGLT1 antibody [N3C3] (NBP2-20338) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
SGLT1/SLC5A1 Antibody - Azide Free

Western Blot: SGLT1/SLC5A1 Antibody [NBP2-20338] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SGLT1 antibody [N3C3] (NBP2-20338) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SGLT1/SLC5A1 Antibody - Azide Free

Western Blot: SGLT1/SLC5A1 Antibody - Azide Free [NBP2-20338] -

Proximal tubule gluconeogenesis and glucose transporters—(A) Glucose concentration in the medium from aliquots of PT-enriched suspensions (n = 6–7/mice/sex/genotype undergoing three potential ex vivo treatments in duplicate) incubated for 1 h with gluconeogenic substrates in the presence or absence of gluconeogenic stimulators, i.e., cyclic AMP and dexamethasone or insulin; * indicates a significant difference between groups (by three-way ANOVA with repeated measures for treatment); (B) Western blots of cortex homogenates (n = 6/group) probed with antibodies against the three rate-limiting enzymes in gluconeogenesis (PEPCK, FBP1, and G6PC), the sodium glucose cotransporters (SGLT1 and SGLT2), and the sodium-coupled neutral amino acid transporter type 3 (SNAT3); (C) summary (mean +/- sem) of the Ponceau-Red-stain-normalized band densities for each of the blots with the mean of wild-type males (WTMs) set to 1.0; letters above bars indicate results of Tukey’s multiple comparison test (only conducted when a main factor < 0.05) with “A" assigned to the highest mean and all means not different from it, followed by “B", etc. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37175762), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for SGLT1/SLC5A1 Antibody - Azide Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

Azide Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SGLT1/SLC5A1

Glucose transporters are integral membrane proteins that mediate the transport of glucose and structurally-related substances across cellular membranes. Two families of glucose transporter have been identified: the facilitated-diffusion glucose transporter family (GLUT family), also known as 'uniporters,' and the sodium-dependent glucose transporter family (SGLT family), also known as 'cotransporters' or 'symporters' (Wright et al., 1994 [PubMed 7823022]). The SLC5A1 gene encodes a protein that is involved in the active transport of glucose and galactose into eukaryotic and some prokaryotic cells.[supplied by OMIM]

Long Name

Sodium/Glucose Transporter 1

Alternate Names

NAGT, SLC5A1

Gene Symbol

SLC5A1

UniProt

Additional SGLT1/SLC5A1 Products

Product Documents for SGLT1/SLC5A1 Antibody - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SGLT1/SLC5A1 Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Citations for SGLT1/SLC5A1 Antibody - Azide Free

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Protocols

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