SGLT1/SLC5A1 Antibody - Azide Free
Novus Biologicals | Catalog # NBP2-20338
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Predicted:
Canine (90%), Rabbit (90%), Sheep (90%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot, IF/ICC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human SGLT1/SLC5A1. The exact sequence is proprietary.
Reactivity Notes
Zebrafish (80%), Cat (89%), Pig (88%), Chicken (82%), Bovine (89%).
Localization
Membrane; Multi-pass membrane protein
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SGLT1/SLC5A1 Antibody - Azide Free
Immunocytochemistry/ Immunofluorescence: SGLT1/SLC5A1 Antibody [NBP2-20338]
Immunocytochemistry/Immunofluorescence: SGLT1/SLC5A1 Antibody [NBP2-20338] - Analysis of methanol-fixed A431, using SGLT1antibody at 1:500 dilution.Immunohistochemistry-Paraffin: SGLT1/SLC5A1 Antibody [NBP2-20338]
Immunohistochemistry-Paraffin: SGLT1/SLC5A1 Antibody [NBP2-20338] - Human lung fibrose tissue, using SGLT1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.Western Blot: SGLT1/SLC5A1 Antibody [NBP2-20338] -
Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SGLT1 antibody [N3C3] (NBP2-20338) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Western Blot: SGLT1/SLC5A1 Antibody [NBP2-20338] -
Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SGLT1 antibody [N3C3] (NBP2-20338) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: SGLT1/SLC5A1 Antibody - Azide Free [NBP2-20338] -
Proximal tubule gluconeogenesis and glucose transporters—(A) Glucose concentration in the medium from aliquots of PT-enriched suspensions (n = 6–7/mice/sex/genotype undergoing three potential ex vivo treatments in duplicate) incubated for 1 h with gluconeogenic substrates in the presence or absence of gluconeogenic stimulators, i.e., cyclic AMP and dexamethasone or insulin; * indicates a significant difference between groups (by three-way ANOVA with repeated measures for treatment); (B) Western blots of cortex homogenates (n = 6/group) probed with antibodies against the three rate-limiting enzymes in gluconeogenesis (PEPCK, FBP1, and G6PC), the sodium glucose cotransporters (SGLT1 and SGLT2), and the sodium-coupled neutral amino acid transporter type 3 (SNAT3); (C) summary (mean +/- sem) of the Ponceau-Red-stain-normalized band densities for each of the blots with the mean of wild-type males (WTMs) set to 1.0; letters above bars indicate results of Tukey’s multiple comparison test (only conducted when a main factor < 0.05) with “A" assigned to the highest mean and all means not different from it, followed by “B", etc. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37175762), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for SGLT1/SLC5A1 Antibody - Azide Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Frozen
Assay dependent
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Format
Azide Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SGLT1/SLC5A1
Long Name
Sodium/Glucose Transporter 1
Alternate Names
NAGT, SLC5A1
Gene Symbol
SLC5A1
UniProt
Additional SGLT1/SLC5A1 Products
Product Documents for SGLT1/SLC5A1 Antibody - Azide Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SGLT1/SLC5A1 Antibody - Azide Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Related Research Areas
Citations for SGLT1/SLC5A1 Antibody - Azide Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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