SH3BGRL Antibody (OTI8F7)
Novus Biologicals | Catalog # NBP3-45012
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI8F7
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Product Specifications
Immunogen
Full length human recombinant protein of human SH3BGRL (NP_003013) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for SH3BGRL Antibody (OTI8F7)
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Western Blot:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Western Blot:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]
Immunohistochemistry:SH3BGRL Antibody (OTI8F7)[NBP3-45012]![SH3BGRL Antibody (OTI8F7) Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-](https://resources.rndsystems.com/images/products/nbp3-45012_mouse-sh3bgrl-mab-oti8f7-immunohistochemistry-752026224549.jpg "Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-")
Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-SH3BGRL mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.![SH3BGRL Antibody (OTI8F7) Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-](https://resources.rndsystems.com/images/products/nbp3-45012_mouse-sh3bgrl-mab-oti8f7-immunohistochemistry-75202622443312.jpg "Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-")
Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SH3BGRL mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.![SH3BGRL Antibody (OTI8F7) Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-](https://resources.rndsystems.com/images/products/nbp3-45012_mouse-sh3bgrl-mab-oti8f7-immunohistochemistry-75202622452420.jpg "Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-")
Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SH3BGRL mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.![SH3BGRL Antibody (OTI8F7) Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-](https://resources.rndsystems.com/images/products/nbp3-45012_mouse-sh3bgrl-mab-oti8f7-immunohistochemistry-7520262331521.jpg "Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-")
Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SH3BGRL mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.![SH3BGRL Antibody (OTI8F7) Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-](https://resources.rndsystems.com/images/products/nbp3-45012_mouse-sh3bgrl-mab-oti8f7-immunohistochemistry-752026239410.jpg "Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-")
Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-SH3BGRL mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.![SH3BGRL Antibody (OTI8F7) Western Blot;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-](https://resources.rndsystems.com/images/products/nbp3-45012_mouse-sh3bgrl-mab-oti8f7-western-blot-7520262375924.jpg "Western Blot;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-")
Western Blot;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-
HEK293T cells were transfected with the pCMV6-ENTRY control ( Left lane) or pCMV6-ENTRY SH3BGRL ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH3BGRL (1:2000). Positive lysates![SH3BGRL Antibody (OTI8F7) Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-](https://resources.rndsystems.com/images/products/nbp3-45012_mouse-sh3bgrl-mab-oti8f7-immunohistochemistry-75202622494519.jpg "Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-")
Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SH3BGRL mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.![SH3BGRL Antibody (OTI8F7) Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-](https://resources.rndsystems.com/images/products/nbp3-45012_mouse-sh3bgrl-mab-oti8f7-immunohistochemistry-7520262245249.jpg "Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-")
Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-SH3BGRL mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.![SH3BGRL Antibody (OTI8F7) Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-](https://resources.rndsystems.com/images/products/nbp3-45012_mouse-sh3bgrl-mab-oti8f7-immunohistochemistry-7520262254440.jpg "Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-")
Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SH3BGRL mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.![SH3BGRL Antibody (OTI8F7) Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-](https://resources.rndsystems.com/images/products/nbp3-45012_mouse-sh3bgrl-mab-oti8f7-immunohistochemistry-752026231250.jpg "Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-")
Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SH3BGRL mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.![SH3BGRL Antibody (OTI8F7) Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-](https://resources.rndsystems.com/images/products/nbp3-45012_mouse-sh3bgrl-mab-oti8f7-immunohistochemistry-7520262250198.jpg "Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-")
Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SH3BGRL mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.![SH3BGRL Antibody (OTI8F7) Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-](https://resources.rndsystems.com/images/products/nbp3-45012_mouse-sh3bgrl-mab-oti8f7-immunohistochemistry-75202622452414.jpg "Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-")
Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SH3BGRL mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.![SH3BGRL Antibody (OTI8F7) Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-](https://resources.rndsystems.com/images/products/nbp3-45012_mouse-sh3bgrl-mab-oti8f7-immunohistochemistry-752026234422.jpg "Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-")
Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SH3BGRL mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.![SH3BGRL Antibody (OTI8F7) Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-](https://resources.rndsystems.com/images/products/nbp3-45012_mouse-sh3bgrl-mab-oti8f7-immunohistochemistry-7520262314318.jpg "Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-")
Immunohistochemistry;SH3BGRL Antibody (OTI8F7)[NBP3-45012]-
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SH3BGRL mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.Applications for SH3BGRL Antibody (OTI8F7)
Application
Recommended Usage
Immunohistochemistry
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
>95%
Formulation
PBS (pH 7.3) 1% BSA, 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 degrees C. Avoid freeze/thaw cycles.
Background: SH3BGRL
Alternate Names
MGC117402, SH3 domain binding glutamic acid-rich protein like, SH3 domain-binding glutamic acid-rich-like protein, SH3BGR, SH3-binding domain glutamic acid-rich protein like
Gene Symbol
SH3BGRL
Additional SH3BGRL Products
Product Documents for SH3BGRL Antibody (OTI8F7)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SH3BGRL Antibody (OTI8F7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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