SH3BP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20342

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human SH3BP1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (87%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SH3BP1 Antibody - BSA Free

Western Blot: SH3BP1 Antibody [NBP2-20342]

Western Blot: SH3BP1 Antibody [NBP2-20342]

Western Blot: SH3BP1 Antibody [NBP2-20342] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: SH3BP1 Antibody [NBP2-20342]

Immunohistochemistry-Paraffin: SH3BP1 Antibody [NBP2-20342]

Immunohistochemistry-Paraffin: SH3BP1 Antibody [NBP2-20342] - Paraffin-embedded MDA-MB-157 tumor xenograft. SH3BP1 antibody dilution: 1:250.
SH3BP1 Antibody - BSA Free

Immunohistochemistry-Paraffin: SH3BP1 Antibody [NBP2-20342] -

SH3BP1 antibody detects SH3BP1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
SH3BP1 antibody (NBP2-20342) diluted at 1:500.
br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for SH3BP1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SH3BP1

Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. This protein binds preferentially to the ABL1 proto-oncogene, SRC and GRB2. Shows GAP activity for Rac-related proteins but not for Rho- or Ras-related proteins. It inhibits PDGF-induced membrane ruffling mediated by Rac.

Alternate Names

3BP-1, ARHGAP43, SH3 domain binding protein 1, SH3 domain-binding protein 1, SH3-domain binding protein 1

Gene Symbol

SH3BP1

UniProt

Additional SH3BP1 Products

Product Documents for SH3BP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SH3BP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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