SHARPIN Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-92386

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SHARPIN Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KSNSPPALGPEACPVSLPSPPEASTLKGPPPEADLPRSPGNLTEREELAGSLARAIAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit SHARPIN Antibody - BSA Free (NBP1-92386) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SHARPIN Antibody - BSA Free

Western Blot: SHARPIN Antibody [NBP1-92386]

Western Blot: SHARPIN Antibody [NBP1-92386]

Western Blot: SHARPIN Antibody [NBP1-92386] - Analysis in human cell lines PC-3 and Caco-2 using anti-SHARPIN antibody. Corresponding SHARPIN RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
Immunohistochemistry-Paraffin: SHARPIN Antibody [NBP1-92386]

Immunohistochemistry-Paraffin: SHARPIN Antibody [NBP1-92386]

Immunohistochemistry-Paraffin: SHARPIN Antibody [NBP1-92386] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
SHARPIN Antibody - BSA Free Western Blot: SHARPIN Antibody - BSA Free [NBP1-92386]

Western Blot: SHARPIN Antibody - BSA Free [NBP1-92386]

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Lane 4: Human plasma (IgG/HSA depleted)
Lane 5: Human liver tissue

Applications for SHARPIN Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SHARPIN

SHARPIN may have a role in normal immune development and control of inflammation

Long Name

SHANK-associated RH Domain Interactor

Alternate Names

SIPL1

Entrez Gene IDs

81858 (Human)

Gene Symbol

SHARPIN

UniProt

Q9H0F6

Additional SHARPIN Products

Product Documents for SHARPIN Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SHARPIN Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

NFkB Pathway

Citations for SHARPIN Antibody - BSA Free

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Protocols

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FAQs

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