SHB Antibody (HL1455) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25680

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1455 expressed in HEK293

Format

Azide and BSA Free
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Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SHB. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SHB Antibody (HL1455) - Azide and BSA Free

SHB Antibody (HL1455) - Azide and BSA Free

Western Blot: SHB Antibody (HL1455) - Azide and BSA Free [NBP3-25680] -

Western Blot: SHB Antibody (HL1455) - Azide and BSA Free [NBP3-25680] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SHB antibody [HL1455] (NBP3-25680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
SHB Antibody (HL1455) - Azide and BSA Free

Immunohistochemistry-Paraffin: SHB Antibody (HL1455) - Azide and BSA Free [NBP3-25680] -

Immunohistochemistry-Paraffin: SHB Antibody (HL1455) - Azide and BSA Free [NBP3-25680] - SHB antibody [HL1455] detects SHB protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. SHB stained by SHB antibody [HL1455] (NBP3-25680) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for SHB Antibody (HL1455) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SHB

SHB is an adapter protein which regulates several signal transduction cascades by linking activated receptors to downstream signaling components. It may play a role in angiogenesis by regulating FGFR1, VEGFR2 and PDGFR signaling. It may also play a role in T-cell antigen receptor/TCR signaling, interleukin-2 signaling, apoptosis and neuronal cells differentiation by mediating basic-FGF and NGF-induced signaling cascades, and may also regulate IRS1 and IRS2 signaling in insulin-producing cells.

Long Name

Src Homology 2 Domain Containing Adaptor Protein B

Alternate Names

bA3J10.2, RP11-3J10.8, SH2 domain-containing adapter protein B, SHB (Src homology 2 domain containing) adaptor protein B, SHB adaptor protein (a Src homology 2 protein), Src homology 2 domain containing adaptor protein B

Gene Symbol

SHB

Additional SHB Products

Product Documents for SHB Antibody (HL1455) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SHB Antibody (HL1455) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

VEGF - VEGF R2 Signaling Pathways VEGF - VEGF R2 Signaling Pathway Thumbnail