SHC1 Antibody (6E10) - BSA Free

Name: SHC1 Antibody (6E10) - BSA Free
Brand: Novus Biologicals
SKU: NBP2-89051
Price: 449 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-89051

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Product Specifications
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Canine

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 6E10

Format

BSA Free

View all SHC1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to aa 33-39 (E33LPpSPSA39) of SHC1 phosphorylated at Ser36 conjugated to KLH.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

Thiophilic adsorption and size exclusion chromatography purified.

Applications for SHC1 Antibody (6E10) - BSA Free

Application

Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Western Blot: 1 ug/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 1% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 2 x PBS). Immunoprecipitation of shc prior to detection with MAb to Shc (phosphorylated) (pSer36) (6E10) is recommended.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 1ml water (15 minutes at room temperature).

Formulation

Lyophilized from 1ml of 2x PBS, PEG, and sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: SHC1

Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc andisoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of theGRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoformp52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shcdoes not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular responseto oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and isindispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome crelease and apoptosis. The expression of isoform p66Shc has been correlated with life span

Long Name

SHC-transforming Protein 1

Alternate Names

SHCA

Gene Symbol

SHC1

Additional SHC1 Products

Product Documents for SHC1 Antibody (6E10) - BSA Free

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Certificate of Analysis

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Product Specific Notices for SHC1 Antibody (6E10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.