Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, Simple Western, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all AHR Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human AHR
Asn704-Leu848
Accession # P35869

Specificity

Detects human AHR in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human AHR Antibody

Detection of Human AHR antibody by Western Blot.

Detection of Human AHR by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and PC-3 human prostate cancer cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6185) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for AHR at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

AHR antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

AHR in HeLa Human Cell Line.

AHR was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6185) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of AHR antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of AHR in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6185, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Detection of Human AHR antibody by Simple WesternTM.

Detection of Human AHR by Simple WesternTM.

Simple Western lane view shows lysates of PC-3 human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for AHR at approximately 109 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6185) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Shows Human AHR Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human AHR Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and AHR knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6185) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for AHR at approximately 100 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # 2275-PC-100) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human AHR Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Knockout Validated

AHR is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in AHR knockout HeLa cell line.

Simple Western

10 µg/mL
Sample: PC‑3 human prostate cancer cell line

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and PC‑3 human prostate cancer cell line

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: AHR

AHR (Aryl-hydrocarbon receptor; also known as bHLHE76) is a 110 kDa member of the bHLH/PAS transcription factor family. It is widely expressed (breast, lung, liver), and serves many functions. First, it binds multiple xenobiotic chemicals in the cytoplasm. This induces dimerization with ARNT, translocation to the nucleus, and activation of P450 genes such as CYP1A1 and UGT1A6. Second, it appears to block cell cycle progression, possibly via a down‑regulation of CDK proteins. And third, it blocks apoptosis by interacting with E2F1, thus silencing TP73 and Apaf1 genes. Human AHR is 848 amino acids (aa) in length. It contains a 10 aa prosegment, plus a 838 aa mature molecule that contains a DNA binding motif (aa 13-40), a bHLH region (aa 41-81), and two PAS domains (aa 111-342). Over aa 704-848, human AHR shares 70% aa identity with mouse AHR.

Long Name

Aryl Hydrocarbon Receptor

Alternate Names

BHLHE76

Entrez Gene IDs

196 (Human); 11622 (Mouse); 25690 (Rat)

Gene Symbol

AHR

UniProt

P35869 (Human)

Additional AHR Products

Product Documents for Human AHR Antibody

Certificate of Analysis

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Product Specific Notices for Human AHR Antibody

For research use only

Citations for Human AHR Antibody

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Protocols

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