SHIP2/INPPL1 Antibody (3E6) - Azide and BSA Free

Novus Biologicals | Catalog # H00003636-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Monkey

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3E6

Format

Azide and BSA Free
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Product Specifications

Immunogen

INPPL1 (NP_001558, 1159 a.a. ~ 1258 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PSDYGRPLSFPPPRIRESIQEDLAEEAPCLQGGRASGLGEAGMSAWLRAIGLERYEEGLVHNGWDDLEFLSDITEEDLEEAGVQDPAHKRLLLDTLQLSK

Localization

Cytoplasmic and peripheral membrane

Specificity

INPPL1 - inositol polyphosphate phosphatase-like 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SHIP2/INPPL1 Antibody (3E6) - Azide and BSA Free

Western Blot: SHIP2/INPPL1 Antibody (3E6) [H00003636-M01]

Western Blot: SHIP2/INPPL1 Antibody (3E6) [H00003636-M01]

Western Blot: SHIP2/INPPL1 Antibody (3E6) [H00003636-M01] - INPPL1 monoclonal antibody (M01), clone 3E6 Analysis of INPPL1 expression in HeLa.
ELISA: SHIP2/INPPL1 Antibody (3E6) [H00003636-M01]

ELISA: SHIP2/INPPL1 Antibody (3E6) [H00003636-M01]

ELISA: SHIP2/INPPL1 Antibody (3E6) [H00003636-M01] - SHIP2/INPPL1 Antibody (3E6) [H00003636-M01] - Detection limit for recombinant GST tagged INPPL1 is approximately 0.1ng/ml as a capture antibody.

Applications for SHIP2/INPPL1 Antibody (3E6) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for ELISA. ICC/IF usage was reported in scientific literature.

Reviewed Applications

Read 1 review rated 4 using H00003636-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SHIP2

INPPL1 is a phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways with wide reaching effects It plays a central role in regulation of PI3K-dependent insulin signaling and confers resistance to dietary obesity. It is part of a signaling pathway that regulates actin cytoskeleton remodeling. It regulates cell adhesion and cell spreading and acts as a regulator of neuritogenesis. It acts as a negative regulator of the FC-gamma-RIIA receptor (FCGR2A). and mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. It is involved in the EGF signaling pathway.

Long Name

SH2-containing Inositol-5'-Phosphatase 2

Alternate Names

INPPL1

Entrez Gene IDs

3636 (Human)

Gene Symbol

INPPL1

OMIM

600829 (Human)

UniProt

NP_001558 (Human)

Additional SHIP2 Products

Product Documents for SHIP2/INPPL1 Antibody (3E6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SHIP2/INPPL1 Antibody (3E6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SHIP2/INPPL1 Antibody (3E6) - Azide and BSA Free

Customer Reviews for SHIP2/INPPL1 Antibody (3E6) - Azide and BSA Free (1)

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  • SHIP2/INPPL1 Antibody (3E6.)
    Name: Cedric Asensio
    Application: Western Blot
    Sample Tested: PC12 cell lysate
    Species: Rat
    Verified Customer | Posted 11/01/2017
    PC12 Whole Cell lysate (20ug), probed with 1:200 3E6 mAb SHIP2.
    Used Ab in PBS-T Milk @1:200. Using Goat-Anti-Mouse-HRP as secondary.
    SHIP2/INPPL1 Antibody (3E6) - Azide and BSA Free H00003636-M01

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