SHP-2/PTPN11 Antibody (6G11) - BSA Free

Name: SHP-2/PTPN11 Antibody (6G11) - BSA Free
Brand: Novus Biologicals
SKU: NBP3-26357
Price: 469 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-26357

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6G11 expressed in HEK293

Format

BSA Free

View all SHP-2 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human SHP-2/PTPN11 [UniProt Q06124]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SHP-2/PTPN11 Antibody (6G11) - BSA Free

Immunohistochemistry: SHP-2/PTPN11 Antibody (6G11) [NBP3-26357] -

Immunohistochemistry: SHP-2/PTPN11 Antibody (6G11) [NBP3-26357] - Image of SHP-2/PTPN11 Antibody (6G11) diluted at 1:100 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Western Blot: SHP-2/PTPN11 Antibody (6G11) [NBP3-26357] -

Western Blot: SHP-2/PTPN11 Antibody (6G11) [NBP3-26357] - Positive Western Blot detected in: Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate.
All lanes: SHP-2/PTPN11 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 69, 53 kDa
Observed band size: 69 kDa

Applications for SHP-2/PTPN11 Antibody (6G11) - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: SHP-2

SHP-2 (also called PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp and PTPN11) is a protein tyrosine phosphatase (PTP) expressed in most cell types (unlike SHP-1), (1). SHP-2 is involved in t the signal transduction stimulated by EGF, PDGF and Insulin. After cell stimulation, SHP-2 (as well as SHP-1), translocates from cytosol to the plasma membrane and binds to tyrosine-phosphorylated receptors through their SH2 domains, becoming activated in the process (2). When phosphorylated, SHP-2 has been shown to bind Grb2, EGFR, PDGFR, IRS-1 via its SH2 domain (3). Over expression of SHP-2 leads to Juvenile Myelo-monocytic Leukemia and Noonan syndrome (4-5).

Long Name

Src Homology Region 2 Domain Phosphatase 2

Alternate Names

PTP1D, PTP2C, PTPN11, SH-PTP2, SHP2

Gene Symbol

PTPN11

Additional SHP-2 Products

Product Documents for SHP-2/PTPN11 Antibody (6G11) - BSA Free

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Certificate of Analysis

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Product Specific Notices for SHP-2/PTPN11 Antibody (6G11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.