SIAH1 Antibody

Novus Biologicals | Catalog # NB300-974

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Peptide ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SIAH1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence SRQTATALPTGTSKC corresponding to N-Terminus according to NP_003022.3, NP_001006611.1.

Specificity

This product is expected to recognize both reported isoforms (according to NP_003022.3; NP_001006611.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SIAH1 Antibody

Immunocytochemistry/ Immunofluorescence: SIAH1 Antibody [NB300-974]

Immunocytochemistry/ Immunofluorescence: SIAH1 Antibody [NB300-974]

Immunocytochemistry/Immunofluorescence: SIAH1 Antibody [NB300-974] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing mitochondrial/cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for SIAH1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:128000
Application Notes
Use in immunoprecipitation reported in scientific literature (PMID: 19479934).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SIAH1

This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Alternate Names

E3 ubiquitin-protein ligase SIAH1, EC 6.3.2, EC 6.3.2.-, FLJ08065, hSIAH1, HUMSIAH, Seven in absentia homolog 1, seven in absentia homolog 1 (Drosophila), Siah-1, SIAH1A, Siah-1a

Entrez Gene IDs

6477 (Human); 20437 (Mouse); 140941 (Rat)

Gene Symbol

SIAH1

UniProt

Q8IUQ4 (Human)

Additional SIAH1 Products

Product Documents for SIAH1 Antibody

Certificate of Analysis

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Product Specific Notices for SIAH1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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