SIAH1 Antibody - BSA Free

Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-20356]

Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-20356] - Human lung Papillary adenocarcinoma, using SIAH1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: SIAH1 Antibody [NBP2-20356] -

Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SIAH1 antibody (NBP2-20356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: SIAH1 Antibody - BSA Free [NBP2-20356] -

Investigating Functional Role of Hits in CD133 Regulation and Stemness. (a) Characterization of CD133 surface levels in pooled patient derived GSC BT935 samples after knockouts of candidate genes. (b) Measurements of Mean Fluorescence Intensity of CD133 surface levels in pooled patient derived GSC BT935 samples after knockouts of candidate genes. (c) Immunoblot bands of CD133 and beta-actin (loading control) after pooled knockouts of candidate genes in BT935. Protein quantification of normalization (right) to control AAVS1 lane after knockouts of candidate genes. Original blots/gels are presented in Supplementary Fig. 7 and quantifications provided in Supplementary Table 2. (d) Self-renewal capacity readout of GSC BT935 samples after pooled knockouts of candidate genes (n = 9). (e) Proliferative capacity readout of GSC BT935 samples after knockouts of candidate genes (n = 9). All statistical significance analysis was determined using paired t-tests. The p-values of biological processes are represented by; ns (not significant); p < 0.05 (*), p < 0.01 (**), p < 0.001 (***). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/41102523), licensed under a CC-BY license. Not internally tested by Novus Biologicals.