Siglec-1/CD169 Antibody (7-239) - BSA Free

Novus Biologicals | Catalog # NBP2-37729

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7-239

Format

BSA Free
View all Siglec-1/CD169 Primary Antibodies »

Product Specifications

Immunogen

Human rhinovirus 14-infected monocyte-derived dendritic cells.

Specificity

The mouse monoclonal antibody 7-239 recognizes CD169 (sialoadhesin, Siglec-1), a 210 kDa type I transmembrane glycoprotein expressed on macrophages and dendritic cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

210 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Siglec-1/CD169 Antibody (7-239) - BSA Free

Flow Cytometry: Siglec-1/CD169 Antibody (7-239) - BSA Free [NBP2-37729]

Flow Cytometry: Siglec-1/CD169 Antibody (7-239) - BSA Free [NBP2-37729]

Flow Cytometry: Siglec-1/CD169 Antibody (7-239) [NBP2-37729] - Staining of CD169 on buffy coat diff. monocytes by anti-CD169 (7-239) PE.

Applications for Siglec-1/CD169 Antibody (7-239) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Blocking/Neutralizing: inhibition of erythrocyte rosetting with cells expressing cd169. This antibody is CyTOF ready.

Reviewed Applications

Read 1 review rated 5 using NBP2-37729 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Siglec-1/CD169

The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumours.

Long Name

Sialic Acid Binding Ig-like Lectin 1

Alternate Names

CD169, Siglec1

Gene Symbol

SIGLEC1

Additional Siglec-1/CD169 Products

Product Documents for Siglec-1/CD169 Antibody (7-239) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Siglec-1/CD169 Antibody (7-239) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Siglec-1/CD169 Antibody (7-239) - BSA Free (1)

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  • Siglec-1/CD169 Antibody (7-239)
    Name: Jason Hammonds
    Application: Immunocytochemistry
    Sample Tested: Primary human macrophages
    Species: Human
    Verified Customer | Posted 03/23/2017
    IF staining of Siglec-1 in GMCSF matured, primary human macrophages at 1:200. DAKO blocking and background reducing antibody diluent. DAPI nuclear stain.
    Siglec-1/CD169 Antibody (7-239) - BSA Free NBP2-37729

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