Simple Plex Discovery Human Syndecan-1 Assay New

Catalog #: SPCKB-PS-014654 Datasheet / COA / SDS

Simple Plex Human Syndecan-1 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-014654
SPCKA-PS-014654
SPCKC-PS-014654

Control Products Available

897218: Simple Plex Discovery Control for Human Syndecan-1

Simple Plex Discovery Human Syndecan-1 Assay Standard Curve

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Discovery Human Syndecan-1 Assay Summary

Assay Type

utomated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Urine (10 uL)

Sensitivity

0.61 pg/mL

Assay Range

5.2 - 20,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Specificity

Natural and recombinant human Syndecan-1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex Discovery assay is for the detection of human Syndecan-1 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Syndecan-1 assay

Inter-Assay Precision (Precision between assays) Replicates of each Syndecan-1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Syndecan-1

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	36	36
Mean (pg/mL)	158	8446	155	7808
Standard Deviation	6.08	526	11.7	717
CV%	3.8	6.2	7.5	9.2

Recovery

Recovery for the human Syndecan-1 assays was evaluated at three different spiked concentrations of Syndecan-1 protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	103	102-103

Linearity

Samples containing and/or spiked with high concentrations of Syndecan-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Simple Plex Discovery Human Syndecan-1 Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human Syndecan-1 Assay. Assay range is 5.24-20,000 pg/mL.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Syndecan-1/CD138

Syndecans are type 1 transmembrane proteoglycans that constitute the major physiological form of heparan sulfate (HS) on the cell surface. The four vertebrate syndecans, Syndecan-1 through -4, have similar short cytoplasmic domains and extracellular portions that diverge, except for HS attachment sites. Structurally diverse side chains add considerably to the size of the core proteins and serve as binding sites for growth factors, cytokines, and extracellular matrix proteins. Syndecans are present as homodimers or multimers, and are often expressed in developmental and cell type-specific patterns. They have many biological roles including regulating cell growth, differentiation, and adhesion.

Entrez Gene IDs:

6382 (Human); 20969 (Mouse); 100519770 (Porcine); 100338470 (Rabbit)

Alternate Names:

CD138 antigen; CD138; SDC; SDC1; SYND1heparan sulfate proteoglycan fibroblast growth factor receptor; syndecan 1; syndecan proteoglycan 1; syndecan; Syndecan1; Syndecan-1