Simple Plex Discovery Human Syndecan-1 Assay

Name: Simple Plex Discovery Human Syndecan-1 Assay
Brand: R&D Systems
SKU: SPCKB-PS-014654
Price: 400 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-PS-014654

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Product Documents

Simple Plex Human Syndecan-1 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.

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Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Urine (10 µL)

Sensitivity

0.61 pg/mL

Assay Range

5.24-20000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Simple Plex Discovery Human Syndecan-1 Assay Summary

Simple Plex Discovery assay is for the detection of human Syndecan-1 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Syndecan-1/CD138 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

utomated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Syndecan-1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Syndecan-1 assay.

Inter-Assay Precision (Precision between assays) Replicates of each Syndecan-1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Syndecan-1.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	36	36
Mean (pg/mL)	158	8446	155	7808
Standard Deviation	6.08	526	11.7	717
CV%	3.8	6.2	7.5	9.2

Recovery for Simple Plex Discovery Human Syndecan-1 Assay

Recovery for the human Syndecan-1 assays was evaluated at three different spiked concentrations of Syndecan-1 protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	103	102-103

Linearity

Samples containing and/or spiked with high concentrations of Syndecan-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Discovery Human Syndecan-1 Assay

Simple Plex Discovery Human Syndecan-1 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human Syndecan-1 Assay. Assay range is 5.24-20,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Syndecan-1/CD138

Syndecans are type 1 transmembrane proteoglycans that constitute the major physiological form of heparan sulfate (HS) on the cell surface. The four vertebrate syndecans, Syndecan-1 through -4, have similar short cytoplasmic domains and extracellular portions that diverge, except for HS attachment sites. Structurally diverse side chains add considerably to the size of the core proteins and serve as binding sites for growth factors, cytokines, and extracellular matrix proteins. Syndecans are present as homodimers or multimers, and are often expressed in developmental and cell type-specific patterns. They have many biological roles including regulating cell growth, differentiation, and adhesion.