Simple Plex Human Leptin Cartridge
R&D Systems | Catalog # SPCKB-PS-000309
Customize This Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Leptin Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Leptin assay.
Inter-Assay Precision (Precision between assays) Replicates of each Leptin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Leptin.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 18 | 18 |
| Mean (pg/mL) | 93.2 | 4319 | 94.7 | 4305 |
| Standard Deviation | 8.70 | 143 | 9.70 | 178 |
| CV% | 9.3 | 3.3 | 10.2 | 4.7 |
Recovery for Simple Plex Human Leptin Cartridge
Recovery was not necessary as human Leptin shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of human Leptin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human Leptin Cartridge
Simple Plex Human Leptin Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Leptin Assay. Assay range is 9.05-13,810 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: Leptin/OB
Leptin, also known as OB, is a polypeptide hormone that regulates adipose tissue mass and energy balance. In mice (ob/ob) and humans, inactivating mutations of the OB gene can cause obesity. Leptin signals through the transmembrane Leptin R in the hypothalamus but can also bind shorter and soluble forms of Leptin R which do not signal. Adipocytes increase Leptin expression as cell size increases, resulting in depressed appetite and increased energy expenditure. Low Leptin levels prompt increased appetite and decreased energy expenditure. Leptin can also block the onset of puberty or menses during starvation, extreme exercise-induced weight loss, anorexia, or cancer-induced cachexia.
Additional Leptin/OB Products
Product Documents for Simple Plex Human Leptin Cartridge
Certificate of Analysis
To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.
Product Specific Notices for Simple Plex Human Leptin Cartridge
For research use only
Citations for Simple Plex Human Leptin Cartridge
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Associated Pathways
Mesenchymal Stem Cell Differentiation Pathways & Lineage-specific Markers
