Simple Plex Human SCF/c-kit Ligand Cartridge

R&D Systems | Catalog # SPCKB-PS-000526

Simple Plex Human SCF/c-kit Ligand assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (5 µL), EDTA Plasma (5 µL), Heparin Plasma (5 µL)

Sensitivity

7.3 pg/mL

Assay Range

59.1-225520 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human SCF/c-kit Ligand Cartridge Summary

Simple Plex assay for the detection of human Stem Cell Factor (SCF) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all SCF/c-kit Ligand Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human SCF.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 17
Mean (pg/mL) 1166 60,559 1351 69,609
Standard Deviation 55.1 3209 102 5219
CV% 4.7 5.3 7.5 7.5

Recovery for Simple Plex Human SCF/c-kit Ligand Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 99 95-104
EDTA Plasma (n=4) 100 95-112
Heparin Plasma (n=4) 102 95-109
Serum (n=4) 104 96-109

Linearity

Samples containing and/or spiked with high concentrations of human SCF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Human SCF Simple Plex Assay Linearity

Scientific Data Images for Simple Plex Human SCF/c-kit Ligand Cartridge

Human SCF Simple Plex Assay Standard Curve

Human SCF Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SCF/c-kit Ligand

SCF (Stem cell factor), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF) promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. SCF is expressed as a transmembrane, but a soluble form can be released by proteolytic shedding. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.

Long Name

Stem Cell Factor

Alternate Names

c-kit Ligand, DCUA, DFNA69, FPH2, FPHH, KITLG, KL-1, MGF, SHEP7, SLF

Entrez Gene IDs

4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline)

Gene Symbol

KITLG

Additional SCF/c-kit Ligand Products

Product Documents for Simple Plex Human SCF/c-kit Ligand Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human SCF/c-kit Ligand Cartridge

For research use only

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