Simple Plex Mouse IL-5 Cartridge
Simple Plex Mouse IL-5 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Mouse Cell Culture Supernates, Mouse Serum, Mouse EDTA Plasma, Mouse Heparin Plasma
|Intra-Assay Precision||Inter-Assay Precision|
Recovery at three different spiked concentrations within the range of the assay was evaluated.
|Sample Type||Average % Recovery||Range %|
|Mouse EDTA Plasma (n=5)||109||98-120|
|Mouse Heparin Plasma (n=5)||107||98-111|
|Mouse Serum (n=3)||106||101-115|
Mouse IL-5 Ella Assay Standard Curve
Preparation and Storage
IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability.
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