SIN3A Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-38564
![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]](https://beta-resources.rndsystems.com/images/products/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38564-img0011.jpg "Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]")
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Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Validated:
Human
Predicted:
Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: SDDPVEAERWSDYVERYMNSDTTSPELREHLAQKPVFLPRNLRRIRKCQRGREQQEKEGKEGNSKKTMENVDSLDKLECRFKLNSYKMVYVIKSEDYMYRRTALLRAHQSHERVSK
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit SIN3A Antibody - BSA Free (NBP2-38564) is a polyclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for SIN3A Antibody - BSA Free
Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]
Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564] - Staining of human cerebellum shows moderate nuclear positivity in all layers.![Immunohistochemistry: SIN3A Antibody [NBP2-38564]](https://beta-resources.rndsystems.com/images/products/SIN3A-Antibody-Immunohistochemistry-NBP2-38564-img0002.jpg "Immunohistochemistry: SIN3A Antibody [NBP2-38564]")
Immunohistochemistry: SIN3A Antibody [NBP2-38564]
Immunohistochemistry: SIN3A Antibody [NBP2-38564] - Staining of human testis shows nuclear positivity in seminiferus ducts.![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]](https://beta-resources.rndsystems.com/images/products/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38564-img0003.jpg "Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]")
Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]
Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564] - Staining of human liver shows low expression as expected.![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]](https://beta-resources.rndsystems.com/images/products/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38564-img0004.jpg "Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]")
![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]](https://beta-resources.rndsystems.com/images/products/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38564-img0006.jpg "Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]")
Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]
Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564] - Staining of human skin.![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]](https://beta-resources.rndsystems.com/images/products/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38564-img0007.jpg "Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]")
Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]
Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564] - Staining of human kidney.![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]](https://beta-resources.rndsystems.com/images/products/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38564-img0008.jpg "Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]")
Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]
Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564] - Staining of human cerebellum, kidney, liver and testis using Anti-SIN3A antibody NBP2-38564 (A) shows similar protein distribution across tissues to independent antibody NBP2-38949 (B).![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]](https://beta-resources.rndsystems.com/images/products/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38564-img0009.jpg "Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]")
Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]
Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]](https://beta-resources.rndsystems.com/images/products/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38564-img0010.jpg "Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]")
Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]
Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564] - Staining of human kidney shows moderate to strong nuclear positivity in cells in tubules.![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]](https://beta-resources.rndsystems.com/images/products/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38564-img0012.jpg "Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]")
Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564]
Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564] - Staining of human liver shows no positivity in hepatocytes as expected.Applications for SIN3A Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SIN3A
Long Name
SIN3 Homolog A
Alternate Names
DKFZp434K2235, FLJ90319, Histone deacetylase complex subunit Sin3a, KIAA0700, paired amphipathic helix protein Sin3a, SIN3 homolog A, transcription regulator (yeast), SIN3 homolog A, transcriptional regulator (yeast), Transcriptional corepressor Sin3a, transcriptional co-repressor Sin3A, transcriptional regulator, SIN3A
Gene Symbol
SIN3A
UniProt
Additional SIN3A Products
Product Documents for SIN3A Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SIN3A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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