Sirtuin 1/SIRT1 Antibody (7B7) - Azide and BSA Free

Novus Biologicals | Catalog # H00023411-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 7B7

Format

Azide and BSA Free
View all Sirtuin 1/SIRT1 Primary Antibodies »

Product Specifications

Immunogen

SIRT1 (AAH12499, 456 a.a. ~ 555 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESEIEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS

Reactivity Notes

Human. Other species not tested.

Localization

Nuclear

Specificity

SIRT1 - sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Sirtuin 1/SIRT1 Antibody (7B7) - Azide and BSA Free

Western Blot: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01]

Western Blot: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01]

Western Blot: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01] - SIRT1 monoclonal antibody (M01), clone 7B7 Analysis of SIRT1 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01]

Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01]

Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01] - Analysis of monoclonal antibody to SIRT1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01]

Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01]

Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01] - Analysis of monoclonal antibody to SIRT1 on formalin-fixed paraffin-embedded human lateral ventricle wall. Antibody concentration 1 ug/ml.
Sandwich ELISA: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01] - Detection limit for recombinant GST tagged SIRT1 is approximately 0.1ng/ml as a capture antibody.

Applications for Sirtuin 1/SIRT1 Antibody (7B7) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Sirtuin 1/SIRT1

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family.

Long Name

Silent Mating Type Information Regulation 2 Homolog

Alternate Names

SIR2-like protein 1, SIR2alpha, SIR2L1, SIRT1

Entrez Gene IDs

23411 (Human)

Gene Symbol

SIRT1

OMIM

604479 (Human)

UniProt

AAH12499 (Human)

Additional Sirtuin 1/SIRT1 Products

Product Documents for Sirtuin 1/SIRT1 Antibody (7B7) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Sirtuin 1/SIRT1 Antibody (7B7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Sirtuin 1/SIRT1 Antibody (7B7) - Azide and BSA Free

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Protocols

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FAQs

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