Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) [mFluor Violet 610 SE]

Novus Biologicals | Catalog # NBP3-08293MFV610

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

mFluor Violet 610 SE (Excitation = 421 nm, Emission = 613 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # PCRP-SIRT2-1A8
View all Sirtuin 2/SIRT2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Sirtuin 2/SIRT2 protein (Uniprot: Q8IXJ6 )

Localization

Cytoplasm > cytoskeleton. Colocalizes with microtubules

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) [mFluor Violet 610 SE]

Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) [mFluor Violet 610 SE]

Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) [mFluor Violet 610 SE] [NBP3-08293MFV610] -

Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) [mFluor Violet 610 SE] [NBP3-08293MFV610] - Vial of mFluor Violet 610 conjugated antibody. mFluor Violet 610 is optimally excited at 421 nm by the Violet laser (405 nm) and has an emission maximum of 613 nm.

Applications for Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) [mFluor Violet 610 SE]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Protein Array

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Sirtuin 2/SIRT2

Silent information regulator 2 (Sir2) family of enzymes has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging. O-acetyl-ADP-ribose causes a delay/block in oocyte maturation and results in a delay/block in embryo cell division in blastomeres. It has been demonstrated that the production of O-acetyl-ADP-ribose is evolutionarily conserved among Sir2-like enzymes from yeast, Drosophila, and human. Also, endogenous yeast Sir2 complex from telomeres was shown to generate O-acetyl-ADP-ribose (1). SIRT2 is a predominantly cytoplasmic protein that colocalizes with microtubules. SIRT2 deacetylates lysine-40 of alpha-tubulin both in vitro and in vivo. Knockdown of SIRT2 via siRNA results in tubulin hyperacetylation. SIRT2 colocalizes and interacts in vivo with HDAC6, another tubulin deacetylase (2). Cytoskeleton-related protein, SIRT2 is down-regulated in gliomas, and data suggests that ectopic expression of SIRT2 in glioma cell lines led to the perturbation of the microtubule network (3).

Long Name

Silent Mating Type Information Regulation 2 Homolog

Alternate Names

SIR2L2, SIRT2

Gene Symbol

SIRT2

Additional Sirtuin 2/SIRT2 Products

Product Documents for Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) [mFluor Violet 610 SE]

Certificate of Analysis

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Product Specific Notices for Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) [mFluor Violet 610 SE]

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy
Epigenetics
Redox Enzymes

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