Sirtuin 2/SIRT2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87039

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Sirtuin 2/SIRT2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KDKGLLLRCYTQNIDTLERIAGLEQEDLVEAHGTFYTSHCVSASCRHEYPLSWMKEKIFSEVTPKCEDCQSLVKPDIVFFGESLPARFFSCMQSDFLKVDLLL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (83%). Human reactivity reported in scientific literature (PMID: 24469059).

Marker

Early Oligodendrocyte Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Sirtuin 2/SIRT2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Sirtuin 2/SIRT2 Antibody [NBP1-87039]

Immunocytochemistry/ Immunofluorescence: Sirtuin 2/SIRT2 Antibody [NBP1-87039]

Immunocytochemistry/Immunofluorescence: Sirtuin 2/SIRT2 Antibody [NBP1-87039] - Staining of human cell line U-251 MG shows localization to nucleoli, plasma membrane and cytosol. Antibody stainign is shown in green.
Immunohistochemistry-Paraffin: Sirtuin 2/SIRT2 Antibody [NBP1-87039]

Immunohistochemistry-Paraffin: Sirtuin 2/SIRT2 Antibody [NBP1-87039]

Immunohistochemistry-Paraffin: Sirtuin 2/SIRT2 Antibody [NBP1-87039] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Sirtuin 2/SIRT2 Antibody [NBP1-87039]

Immunohistochemistry-Paraffin: Sirtuin 2/SIRT2 Antibody [NBP1-87039]

Immunohistochemistry-Paraffin: Sirtuin 2/SIRT2 Antibody [NBP1-87039] - Staining of human cerebellum shows moderate cytoplasmic positivity in oligodendrocytes.
Immunohistochemistry-Paraffin: Sirtuin 2/SIRT2 Antibody [NBP1-87039]

Immunohistochemistry-Paraffin: Sirtuin 2/SIRT2 Antibody [NBP1-87039]

Immunohistochemistry-Paraffin: Sirtuin 2/SIRT2 Antibody [NBP1-87039] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in oligodendrocytes.
Sirtuin 2/SIRT2 Antibody - BSA Free Immunohistochemistry-Paraffin: Sirtuin 2/SIRT2 Antibody - BSA Free [NBP1-87039]

Immunohistochemistry-Paraffin: Sirtuin 2/SIRT2 Antibody - BSA Free [NBP1-87039]

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Sirtuin 2/SIRT2 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: Sirtuin 2/SIRT2 Antibody - BSA Free [NBP1-87039]

Chromatin Immunoprecipitation-exo-Seq: Sirtuin 2/SIRT2 Antibody - BSA Free [NBP1-87039]

ChIP-Exo-Seq composite graph for Anti-SIRT2 (NBP1-87039) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for Sirtuin 2/SIRT2 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. WB reported in scientific literature (PMID: 24469059).

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sirtuin 2/SIRT2

Silent information regulator 2 (Sir2) family of enzymes has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging. O-acetyl-ADP-ribose causes a delay/block in oocyte maturation and results in a delay/block in embryo cell division in blastomeres. It has been demonstrated that the production of O-acetyl-ADP-ribose is evolutionarily conserved among Sir2-like enzymes from yeast, Drosophila, and human. Also, endogenous yeast Sir2 complex from telomeres was shown to generate O-acetyl-ADP-ribose (1). SIRT2 is a predominantly cytoplasmic protein that colocalizes with microtubules. SIRT2 deacetylates lysine-40 of alpha-tubulin both in vitro and in vivo. Knockdown of SIRT2 via siRNA results in tubulin hyperacetylation. SIRT2 colocalizes and interacts in vivo with HDAC6, another tubulin deacetylase (2). Cytoskeleton-related protein, SIRT2 is down-regulated in gliomas, and data suggests that ectopic expression of SIRT2 in glioma cell lines led to the perturbation of the microtubule network (3).

Long Name

Silent Mating Type Information Regulation 2 Homolog

Alternate Names

SIR2L2, SIRT2

Gene Symbol

SIRT2

Additional Sirtuin 2/SIRT2 Products

Product Documents for Sirtuin 2/SIRT2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Sirtuin 2/SIRT2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy
Epigenetics
Redox Enzymes

Citations for Sirtuin 2/SIRT2 Antibody - BSA Free

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Protocols

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