SLC12A3 [p Thr53] Antibody - Azide Free

Novus Biologicals | Catalog # NBP2-60775

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Predicted:

Guinea Pig (100%), Hamster (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all SLC12A3 Primary Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 conjugated to KLH. Accession # P59158

Modification

p Thr53

Specificity

Specific for endogenous levels of the ~160 kDa SLC12A3 protein phosphorylated at Thr53. Band of interest smearing likely due to glycosylation. Immunolabeling is completely eliminated by treatment with l-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

160 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SLC12A3 [p Thr53] Antibody - Azide Free

Western Blot: SLC12A3 [p Thr53] Antibody [NBP2-60775]

Western Blot: SLC12A3 [p Thr53] Antibody [NBP2-60775]

Western Blot: SLC12A3 [p Thr53] Antibody [NBP2-60775] - Western blot of mouse kidney lysate showing specificimmunolabeling of the ~160 kDa NCC protein phosphorylatedat Thr53 in the first lane (-). Phosphospecificity is shown in thesecond lane (+) where immunolabeling is completelyeliminated by blot treatment with lambda phosphatase (Lambda-Ptase, 1200 units for 30 min)

Applications for SLC12A3 [p Thr53] Antibody - Azide Free

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:10000

Western Blot

1:1000-1:6000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SLC12A3

The SLC12A3 gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman s

Long Name

Solute Carrier Family 12 (Sodium/Chloride Transporters) Member 3

Alternate Names

NCC, NCCT, TSC

Gene Symbol

SLC12A3

Additional SLC12A3 Products

Product Documents for SLC12A3 [p Thr53] Antibody - Azide Free

Certificate of Analysis

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Product Specific Notices for SLC12A3 [p Thr53] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SLC12A3 [p Thr53] Antibody - Azide Free

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Protocols

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FAQs

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