SLC16A3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81251

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoblotting, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SLC16A3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AEEEKLHKPPADSGVDLREVEHFLKAEPEKNGEVVHTPETSV

Reactivity Notes

Rat (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SLC16A3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SLC16A3 Antibody [NBP1-81251]

Immunocytochemistry/ Immunofluorescence: SLC16A3 Antibody [NBP1-81251]

Immunocytochemistry/Immunofluorescence: SLC16A3 Antibody [NBP1-81251] - Staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251]

Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251]

Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251]

Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251]

Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human lung shows strong membranous positivity in macrophages.
Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251]

Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251]

Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251]

Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251]

Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.
Simple Western: SLC16A3 Antibody [NBP1-81251]

Simple Western: SLC16A3 Antibody [NBP1-81251]

Simple Western: SLC16A3 Antibody [NBP1-81251] - Simple Western: SLC16A3 Antibody [NBP1-81251] - Electropherogram image(s) of corresponding Simple Western lane view. SLC16A3 antibody was used at 1:250 dilution on U-251MG lysates(s)
Simple Western: SLC16A3 Antibody [NBP1-81251]

Simple Western: SLC16A3 Antibody [NBP1-81251]

Simple Western: SLC16A3 Antibody [NBP1-81251] - Simple Western: SLC16A3 Antibody [NBP1-81251] - Electropherogram image(s) of corresponding Simple Western lane view. SLC16A3 antibody was used at 1:250 dilution on HeLa lysates(s).

Applications for SLC16A3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:250
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:250, apparent MW was 53 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 1 review rated 5 using NBP1-81251 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SLC16A3

Monocarboxylates, such as lactate and pyruvate, play an integral role in cellular metabolism. Lactic acid is produced in large quantities as a result of glycolysis, which provides the majority of ATP to cells under normal physiological conditions. However, accumulation of lactic acid leads to a decrease in intracellular pH and cessation of glycolysis. In order for glycolysis to continue at a high rate, lactic acid must be transported out of the cell. This transport process is carried out by a family of monocarboxylate transporters (MCTs),which function as proton symports and are stereoselective for L-lactate.The MCT family consists of at least eight members, MCT1-8, which contain between 10-12 transmembrane-helical (TM) domains, with the amino and carboxy termini located in the cytoplasm. MCT1 is widely expressed and is the major form of MCTs in tumor cells and erythrocytes. MCT2 is highly expressed in liver and testis, while MCT3 and MCT4 are predominantly expressed in skeletal muscle.

Long Name

Solute Carrier Family 16 Member 3

Alternate Names

MCT3, MCT4

Entrez Gene IDs

9123 (Human); 80878 (Rat)

Gene Symbol

SLC16A3

UniProt

O15427 (Human)

Additional SLC16A3 Products

Product Documents for SLC16A3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SLC16A3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Citations for SLC16A3 Antibody - BSA Free

Customer Reviews for SLC16A3 Antibody - BSA Free (1)

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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested:
    Species: Human
    Verified Customer | Posted 06/04/2015
    Immunoblot assay of MCT4 expression in human breast cancer cell lines
    SLC16A3 Antibody - BSA Free NBP1-81251

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Protocols

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FAQs for SLC16A3 Antibody - BSA Free

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  • Q: Does the SLC16A3 Antibody (NBP1-81251) also work on frozen sections?

    A: Thank you for contacting Novus Biologicals Technical support. NBP1-81251 has not yet been tested or validated for the use on frozen sections. It is validated and guaranteed to work in IHC on paraffin embedded tissues.

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