SLC28A3 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-29615
![Western Blot: SLC28A3 Antibody [NBP2-29615]](https://resources.rndsystems.com/images/products/SLC28A3-Antibody-Western-Blot-NBP2-29615-img0001.jpg "Western Blot: SLC28A3 Antibody [NBP2-29615]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Primate (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
A synthetic peptide made to an internal portion of the human SLC28A3 protein (between residues 30-100) [NCBI NP_071410.1]
Localization
Multipass transmembrane protein with Cytoplasmic, Helical and Extracellular domains
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SLC28A3 Antibody - BSA Free
Western Blot: SLC28A3 Antibody [NBP2-29615]
Western Blot: SLC28A3 Antibody [NBP2-29615] - Western blot analysis of SLC28A3 in A. PC3 cell lysate B. PC3 lysate with blocking peptide.![Immunohistochemistry-Paraffin: SLC28A3 Antibody [NBP2-29615]](https://resources.rndsystems.com/images/products/SLC28A3-Antibody-Immunohistochemistry-Paraffin-NBP2-29615-img0002.jpg "Immunohistochemistry-Paraffin: SLC28A3 Antibody [NBP2-29615]")
Immunohistochemistry-Paraffin: SLC28A3 Antibody [NBP2-29615]
Immunohistochemistry-Paraffin: SLC28A3 Antibody [NBP2-29615] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human pancreas using SLC28A3 antibody at 1:200 dilution. The antibody generated an expected cytoplasmic staining and the expression was mainly seen in Islet of Langerhans while the pancreatic acinar cells showed very weak staining. Inside the Islet of Langerhans, the alpha and beta cells showed moderate to high staining while the delta cells depicted very intense staining for SLC28A3 protein.Applications for SLC28A3 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
4 ug/ml
Application Notes
SLC28A3 is a multipass membrane protein and its canonical form (Isoform 1) is 691AA long with predicted molecular weight of 76.9 kDa. This SLC28A3 antibody is not expected to detect Isoform 2 of SLC28A3, and in our testing with WB assay, this antibody detected the target band (~75kDa) with one additional/uncharacterized band (~17 kda). Because the immunogen sequence of this SLC28A3 antibody falls within the first cytoplasmic domain of SLC28A3 (see Uniprot# Q9HAS3; aa1-100, the largest cytoplasmic domain of this protein), the expected staining pattern with this antibody would be cytoplasmic in immunostaining assays.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SLC28A3
Alternate Names
CNT 3, CNT3concentrative Na+-nucleoside cotransporter, Concentrative Na(+)-nucleoside cotransporter 3, hCNT3, solute carrier family 28 (sodium-coupled nucleoside transporter), member 3, solute carrier family 28 member 3
Entrez Gene IDs
64078 (Human)
Gene Symbol
SLC28A3
UniProt
Additional SLC28A3 Products
Product Documents for SLC28A3 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SLC28A3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for SLC28A3 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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