SLC6A3/DAT1 Antibody - Azide Free

Novus Biologicals | Catalog # NB300-255

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all SLC6A3/DAT1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acid residues from the extracellular loop 2 region conjugated to KLH. Accession # Q01959

Reactivity Notes

The antibody has been tested in Western blots of SDS-solubilized human striatal samples and in IHC applications with formaldehyde-fixed human and monkey brain sections.

Specificity

Specific for endogenous levels of the ~80 kDa SLC6A3/DAT1 protein. Diffuse band may be observed between ~70-85 kDa due to variable glycosylation.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SLC6A3/DAT1 Antibody - Azide Free

Western Blot: SLC6A3/DAT1 Antibody [NB300-255]

Western Blot: SLC6A3/DAT1 Antibody [NB300-255]

Western Blot: DAT1 Antibody [NB300-255] - Detection of DAT protein in human caudate sample using NB 300-255.
Western Blot: SLC6A3/DAT1 Antibody [NB300-255]

Western Blot: SLC6A3/DAT1 Antibody [NB300-255]

Western Blot: DAT1 Antibody [NB300-255] - Western blot analysis of DAT1 in SDS-solubilized human caudate using NB300-255 at 1:1000.

Applications for SLC6A3/DAT1 Antibody - Azide Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:1000

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SLC6A3/DAT1

Plasmalemmal neurotransmitter transporters sequester synaptic and peri-synaptic transmitter into presynaptic elements. The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. Levels of DAT protein expression are altered by chronic drug administration.

Long Name

Dopamine Transporter

Alternate Names

DAT1, SLC6A3, VNTR

Gene Symbol

SLC6A3

UniProt

Q01959 (Human)

Additional SLC6A3/DAT1 Products

Product Documents for SLC6A3/DAT1 Antibody - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SLC6A3/DAT1 Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Neuronal Markers

Customer Reviews for SLC6A3/DAT1 Antibody - Azide Free

There are currently no reviews for this product. Be the first to review SLC6A3/DAT1 Antibody - Azide Free and earn rewards!

Have you used SLC6A3/DAT1 Antibody - Azide Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for SLC6A3/DAT1 Antibody - Azide Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q: Could you kindly provide published references for Western blot in detection of Dopamine transporter in human brain tissue with your antibody/antibodies?

    A: Catalog numbers NBP2-22164, NB300-254 and NB100-65459 are well characterized DAT1 antibodies that have been referenced. If you refer to our website we have links to the PMID listing for each reference on the individual datasheets.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies