SLC6A4/5-HTTLPR/Serotonin transporter Antibody (9G3) - BSA Free

Novus Biologicals | Catalog # NBP3-15198

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9G3 expressed in HEK293

Format

BSA Free
View all SLC6A4/5-HTTLPR Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human SLC6A4/5-HTTLPR/Serotonin transporter. UniProt ID (P31645).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SLC6A4/5-HTTLPR/Serotonin transporter Antibody (9G3) - BSA Free

Immunohistochemistry: SLC6A4/5-HTTLPR/Serotonin transporter Antibody (9G3) [NBP3-15198]

Immunohistochemistry: SLC6A4/5-HTTLPR/Serotonin transporter Antibody (9G3) [NBP3-15198]

Immunohistochemistry: SLC6A4/5-HTTLPR/Serotonin transporter Antibody (9G3) [NBP3-15198] - IHC image of SLC6A4/5-HTTLPR/Serotonin transporter antibody diluted at 1:100 and staining in paraffin-embedded human testis tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
SLC6A4/5-HTTLPR/Serotonin transporter Antibody (9G3)

Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody (9G3) [NBP3-15198]

Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody (9G3) [NBP3-15198] - Positive WB detected in: U-87 whole cell lysate, 293T whole cell lysate, U-251 whole cell lysate, HepG2 whole cell lysate
All lanes: Serotonin transporter antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 71, 75 kDa
Observed band size: 55 kDa

Applications for SLC6A4/5-HTTLPR/Serotonin transporter Antibody (9G3) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: SLC6A4/5-HTTLPR

Serotonin transporter encodes an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. The encoded protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is a target of psychomotor stimulants, such as amphetamines and cocaine, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, and depression-susceptibility in people experiencing emotional trauma.

Long Name

Solute Carrier Family 6 Member 4

Alternate Names

5-HT Transporter, 5-HTT, 5-HTTLPR, 5HTTLPR, OCD1, Serotonin Transporter 1, SERT

Gene Symbol

SLC6A4

Additional SLC6A4/5-HTTLPR Products

Product Documents for SLC6A4/5-HTTLPR/Serotonin transporter Antibody (9G3) - BSA Free

Certificate of Analysis

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Product Specific Notices for SLC6A4/5-HTTLPR/Serotonin transporter Antibody (9G3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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