Key Product Details

Species Reactivity

Multi-Species

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Sleeping Beauty Transposase Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant Sleeping Beauty Transposase

Specificity

Detects Sleeping Beauty Transposase in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Sleeping Beauty Transposase Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Sleeping Beauty Transposase

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Sleeping Beauty Transposase

SB transposase is the catalytic component of a DNA transposition system that catalyzes the excision of the transposon from its original location and promotes its reintegration elsewhere in the genome. An active transposase that acts on transposon sequences found in vertebrate genomes was reconstructed from consensus sequences of several mutated and inactive Tc1/mariner family transposase genes in salmonid fish. The entire 340 amino aa transposase sequence contains a paired‑like domain with a leucine zipper for DNA binding, a nuclear localization sequence (NLS), a glycine-rich box and a DD(34)E box. The recognition domain for transposon DNA is contained within the first 123 aa of the transposase (1).

References

  1. Ivics, Z. et al. (1997) Cell 91:501.

Additional Sleeping Beauty Transposase Products

Product Documents for Sleeping Beauty Transposase Biotinylated Antibody

Certificate of Analysis

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Product Specific Notices for Sleeping Beauty Transposase Biotinylated Antibody

For research use only

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