Sleeping Beauty Transposase Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (8)
Supplemental Products

Sleeping Beauty Transposase Antibody Summary

Detects Sleeping Beauty Transposase in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant Sleeping Beauty Transposase
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Sleeping Beauty Transposase
5-15 µg/mL
Perfusion fixed paraffin-embedded sections of transgenic mouse intestine expressing Sleeping Beauty transposase subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sleeping Beauty Transposase

SB transposase is the catalytic component of a DNA transposition system that catalyzes the excision of the transposon from its original location and promotes its reintegration elsewhere in the genome. An active transposase that acts on transposon sequences found in vertebrate genomes was reconstructed from consensus sequences of several mutated and inactive Tc1/mariner family transposase genes in salmonid fish. The entire 340 amino aa transposase sequence contains a paired‑like domain with a leucine zipper for DNA binding, a nuclear localization sequence (NLS), a glycine-rich box and a DD(34)E box. The recognition domain for transposon DNA is contained within the first 123 aa of the transposase (1).

  1. Ivics, Z. et al. (1997) Cell 91:501.
Alternate Names
SB Transposase

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Citations for Sleeping Beauty Transposase Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Engineered Sleeping Beauty transposase redirects transposon integration away from genes
    Authors: C Miskey, L Kesselring, I Querques, G Abrusán, O Barabas, Z Ivics
    Nucleic Acids Research, 2022;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Targeting CD33 in Chemoresistant AML Patient-Derived Xenografts by CAR-CIK Cells Modified with an Improved SB Transposon System
    Authors: MC Rotiroti, C Buracchi, S Arcangeli, S Galimberti, MG Valsecchi, VM Perriello, T Rasko, G Alberti, CF Magnani, C Cappuzzell, F Lundberg, A Pande, G Dastoli, M Introna, M Serafini, E Biagi, Z Izsvák, A Biondi, S Tettamanti
    Mol. Ther., 2020;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Regulated complex assembly safeguards the fidelity of Sleeping Beauty transposition
    Nucleic Acids Res, 2016;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  4. Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering
    Authors: F Voigt, L Wiedemann, C Zuliani, I Querques, A Sebe, L Mátés, Z Izsvák, Z Ivics, O Barabas
    Nat Commun, 2016;7(0):11126.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Transposon mutagenesis identifies genes that cooperate with mutant Pten in breast cancer progression
    Proc. Natl. Acad. Sci. U.S.A., 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Transposon mutagenesis identifies genetic drivers of Braf(V600E) melanoma.
    Authors: Mann M, Black M, Jones D, Ward J, Yew C, Newberg J, Dupuy A, Rust A, Bosenberg M, McMahon M, Print C, Copeland N, Jenkins N
    Nat Genet, 2015;47(5):486-95.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. A transposon-based analysis of gene mutations related to skin cancer development.
    Authors: Quintana R, Dupuy A, Bravo A, Casanova M, Alameda J, Page A, Sanchez-Viera M, Ramirez A, Navarro M
    J Invest Dermatol, 2013;133(1):239-48.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Whole-body sleeping beauty mutagenesis can cause penetrant leukemia/lymphoma and rare high-grade glioma without associated embryonic lethality.
    Authors: Collier LS, Adams DJ, Hackett CS, Bendzick LE, Akagi K, Davies MN, Diers MD, Rodriguez FJ, Bender AM, Tieu C, Matise I, Dupuy AJ, Copeland NG, Jenkins NA, Hodgson JG, Weiss WA, Jenkins RB, Largaespada DA
    Cancer Res., 2009;69(21):8429-37.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P


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