Sleeping Beauty Transposase Antibody

R&D Systems | Catalog # AF2798

R&D Systems

Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Sleeping Beauty Transposase Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant Sleeping Beauty Transposase

Specificity

Detects Sleeping Beauty Transposase in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Sleeping Beauty Transposase Antibody

Perfusion fixed paraffin-embedded sections of mouse liver

Sleeping Beauty Transposase was detected in perfusion fixed paraffin-embedded sections of mouse liver using Goat Anti-Sleeping Beauty Transposase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2798) at 0.25 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using the HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Sleeping Beauty Transposase Antibody

Application
Recommended Usage

Immunohistochemistry

0.25-25 µg/mL
Sample: Perfusion fixed paraffin-embedded sections of mouse liver

Western Blot

0.1 µg/mL
Sample: Recombinant Sleeping Beauty Transposase

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Sleeping Beauty Transposase

SB transposase is the catalytic component of a DNA transposition system that catalyzes the excision of the transposon from its original location and promotes its reintegration elsewhere in the genome. An active transposase that acts on transposon sequences found in vertebrate genomes was reconstructed from consensus sequences of several mutated and inactive Tc1/mariner family transposase genes in salmonid fish. The entire 340 amino aa transposase sequence contains a paired‑like domain with a leucine zipper for DNA binding, a nuclear localization sequence (NLS), a glycine-rich box and a DD(34)E box. The recognition domain for transposon DNA is contained within the first 123 aa of the transposase (1).

References

  1. Ivics, Z. et al. (1997) Cell 91:501.

Additional Sleeping Beauty Transposase Products

Product Documents for Sleeping Beauty Transposase Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Sleeping Beauty Transposase Antibody

For research use only

Citations for Sleeping Beauty Transposase Antibody

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Protocols

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