Slit2 Antibody (1T5A3)

Name: Slit2 Antibody (1T5A3)
Brand: Novus Biologicals
SKU: NBP3-16194
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16194

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1T5A3 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1400-1500 of human Slit2 (O94813). GHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Slit2 Antibody (1T5A3)

Western Blot: Slit2 Antibody (1T5A3) [NBP3-16194]

Western Blot: Slit2 Antibody (1T5A3) [NBP3-16194] - Western blot analysis of extracts of various cell lines, using Slit2 Rabbit mAb (NBP3-16194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Immunohistochemistry-Paraffin: Slit2 Antibody (1T5A3) [NBP3-16194]

Immunohistochemistry-Paraffin: Slit2 Antibody (1T5A3) [NBP3-16194] - Immunohistochemistry of paraffin-embedded rat ovary using Slit2 Rabbit mAb (NBP3-16194) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for Slit2 Antibody (1T5A3)

Application

Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Slit2

Slit proteins are ligands at the Roundabout (Robo) receptors and act as guidance cues in axonal migration/navigation during neural development, at the ventral midline of the neural tube. Slit1 and Slit2 are essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. A number of cleavage products are reported in the literature for Slit2 protiens (following alternate splicing). The C-terminal cleavage proteins are more diffusible than the larger N-terminal protein that is more tightly cell associated. Slit2 protein is expressed in fetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea.

Long Name

SLIT Homolog 2

Alternate Names

SLIL3

Gene Symbol

SLIT2

Additional Slit2 Products

Product Documents for Slit2 Antibody (1T5A3)

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Certificate of Analysis

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Product Specific Notices for Slit2 Antibody (1T5A3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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