Slit2 Antibody

Novus Biologicals | Catalog # NB100-40807

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence DDCQDNKCKNGAH corresponding to internal region according to NP_004778.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Novus Biologicals Goat Slit2 Antibody (NB100-40807) is a polyclonal antibody validated for use in IHC and ELISA. Anti-Slit2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Slit2 Antibody

Immunohistochemistry-Paraffin: Slit2 Antibody [NB100-40807]

Immunohistochemistry-Paraffin: Slit2 Antibody [NB100-40807]

Immunohistochemistry-Paraffin: Slit2 Antibody [NB100-40807] - Staining of paraffin embedded Human Spinal Cord. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Applications for Slit2 Antibody

Application
Recommended Usage

Immunohistochemistry

4 - 8 ug/ml

Immunohistochemistry-Paraffin

4 - 8 ug/ml

Peptide ELISA

Detection limit 1:1000
Application Notes
IHC-P: Human Spinal Cord shows staining of the cellular membrane in the bundles of sensory fibres.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Slit2

Slit proteins are ligands at the Roundabout (Robo) receptors and act as guidance cues in axonal migration/navigation during neural development, at the ventral midline of the neural tube. Slit1 and Slit2 are essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. A number of cleavage products are reported in the literature for Slit2 protiens (following alternate splicing). The C-terminal cleavage proteins are more diffusible than the larger N-terminal protein that is more tightly cell associated. Slit2 protein is expressed in fetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea.

Long Name

SLIT Homolog 2

Alternate Names

SLIL3

Entrez Gene IDs

9353 (Human); 20563 (Mouse); 360272 (Rat)

Gene Symbol

SLIT2

UniProt

Additional Slit2 Products

Product Documents for Slit2 Antibody

Certificate of Analysis

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Product Specific Notices for Slit2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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