Slit2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20398

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Bovine (99%), Chicken (97%), Xenopus (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Slit2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Slit2. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (88%), Xenopus laevis (93%).

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

170 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Slit2 Antibody - BSA Free (NBP2-20398) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-Slit2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Slit2 Antibody - BSA Free

Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398]

Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398]

Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] - Human gastric carcinoma. SLIT2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Slit2 Antibody - BSA Free

Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] -

SLIT2 antibody detects SLIT2 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
SLIT2 antibody (NBP2-20398) diluted at 1:400.
br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Slit2 Antibody - BSA Free

Western Blot: Slit2 Antibody [NBP2-20398] -

SLIT2 antibody detects SLIT2 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SLIT2 antibody (NBP2-20398) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Slit2 Antibody - BSA Free

Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] -

SLIT2 antibody detects SLIT2 protein at cytoplasm in mouse fetal brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fetal brain.
Green: SLIT2 antibody (NBP2-20398) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488).
Blue: Nuclear staining with Hoechst 33342.
br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Slit2 Antibody - BSA Free

Western Blot: Slit2 Antibody [NBP2-20398] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SLIT2 antibody (NBP2-20398) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Slit2 Antibody - BSA Free

Western Blot: Slit2 Antibody [NBP2-20398] -

Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SLIT2 antibody (NBP2-20398) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Slit2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Slit2

Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.

Long Name

SLIT Homolog 2

Alternate Names

SLIL3

Gene Symbol

SLIT2

UniProt

O94813

Additional Slit2 Products

Product Documents for Slit2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Slit2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Slit2 Antibody - BSA Free

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Protocols

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FAQs

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