SLP-2 Antibody (OTI1F5) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-74227

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1F5

Format

Azide and BSA Free
View all SLP-2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human STOML2 (NP_038470) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

38.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SLP-2 Antibody (OTI1F5) - Azide and BSA Free

Western Blot: SLP-2 Antibody (OTI1F5)Azide and BSA Free [NBP2-74227]

Western Blot: SLP-2 Antibody (OTI1F5)Azide and BSA Free [NBP2-74227]

Western Blot: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLP-2.
Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227]

Immunohistochemistry: SLP-2 Antibody (OTI1F5) - Azide and BSA Free [NBP2-74227] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)

Applications for SLP-2 Antibody (OTI1F5) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SLP-2

STOML2 is similar in sequence to stomatin. It is a 356 amino acid protein with a calculated molecular mass of 38.5 kDa. STOML2 has 3 potential initiator sites, all sharing the same open reading frame. The STOML2 protein contains the cognate stomatin family consensus sequence, but it lacks the characteristic N-terminal hydrophobic domain and palmitoylation consensus sequence. STOML2 shares greatest sequence homology with stomatin and SLP1 in a region predicted to contain beta sheet and alpha helix structures. Northern blot analysis detected a 1.5 kb STOML2 transcript in all tissues examined, with highest levels in heart, liver, and pancreas. Western blot analysis detected STOML2 at apparent molecular masses of 45.5 kDa or 44.6 kDa in all human and mammalian cell lines and tissues examined, including red blood cells. Some cells also showed a faint band at about 34.3 kDa, which may represent translation from an alternate initiation site.

Alternate Names

EPB72-like protein 2, HSPC108, SLP2, SLP-2stomatin-like protein 2, stomatin (EPB72)-like 2, stomatin-like 2

Gene Symbol

STOML2

Additional SLP-2 Products

Product Documents for SLP-2 Antibody (OTI1F5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SLP-2 Antibody (OTI1F5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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