SLX4 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-28679
![Immunoprecipitation: SLX4 Antibody [NBP1-28679]](https://resources.rndsystems.com/images/products/BTBD12-Antibody-Immunoprecipitation-NBP1-28679-img0002.jpg "Immunoprecipitation: SLX4 Antibody [NBP1-28679]")
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1650 and 1700 of human SLX4 using the numbering given in entry NP_115820.2 (GeneID 84464).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SLX4 Antibody - BSA Free
Immunoprecipitation: SLX4 Antibody [NBP1-28679]
Immunoprecipitation: BTBD12 Antibody [NBP1-28679] - Detection of human BTBD12 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BTBD12 antibody NBP1-28679 used for IP at 6 ug per reaction. BTBD12 was also immunoprecipitated by another rabbit anti-BTBD12 antibody. For blotting immunoprecipitated BTBD12, NBP1-28679 was used at 3 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for SLX4 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
Reported in (PMID: 21655083)
Immunoprecipitation
2 - 10 ug/mg lysate
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SLX4
Long Name
SLX4 Structure-Specific Endonuclease Subunit
Alternate Names
BTBD12, FANCP, KIAA1784, KIAA1987
Entrez Gene IDs
84464 (Human)
Gene Symbol
SLX4
UniProt
Additional SLX4 Products
Product Documents for SLX4 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SLX4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for SLX4 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for SLX4 Antibody - BSA Free
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Q: What concentration do you recommend using this antibody at for probing Westerns?
A: Please note that our product BTBD12 Antibody (NBP1-28679) is recommended for immunoprecipitation (IP), but not for Western blot - the minus sign next to 'WB' on the datasheet denotes 'Western blot - negative' and means that testing has indicated that this product is not suitable for western blot.
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