SMARCA5/SNF2H Antibody (6H8I7)

Novus Biologicals | Catalog # NBP3-16216

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6H8I7 expressed in HEK293
View all SMARCA5/SNF2H Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 953-1052 of human SMARCA5/SNF2H (O60264). RFLICMLHKLGFDKENVYDELRQCIRNSPQFRFDWFLKSRTAMELQRRCNTLITLIERENMELEEKEKAEKKKRGPKPSTQKRKMDGAPDGRGRKKKLKL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMARCA5/SNF2H Antibody (6H8I7)

Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216]

Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216]

Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Western blot analysis of extracts of Mouse thymus, using SMARCA5/SMARCA5/SNF2H Rabbit mAb (NBP3-16216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216]

Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216]

Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Western blot analysis of extracts of various cell lines, using SMARCA5/SMARCA5/SNF2H Rabbit mAb (NBP3-16216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
SMARCA5/SNF2H Antibody (6H8I7)

Immunocytochemistry/ Immunofluorescence: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] -

Immunocytochemistry/ Immunofluorescence: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Confocal imaging of Hep G2 cells using SMARCA5/SNF2H Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
SMARCA5/SNF2H Antibody (6H8I7)

Immunocytochemistry/ Immunofluorescence: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] -

Immunocytochemistry/ Immunofluorescence: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Confocal imaging of C6 cells using SMARCA5/SNF2H Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Applications for SMARCA5/SNF2H Antibody (6H8I7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SMARCA5/SNF2H

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SNF2h/ISWI, also known as WCRF135, is the gene product of SMARCA5 that associates with WSTF (Williams Syndrome transcription factor) and several other nuclear proteins to mediate chromatin remodeling. SNF2h is also part of the NoRC (nucleolar remodeling complex).

Long Name

SWI/SNF-related, Matrix-associated, Actin-dependent Regulator of Chromatin, Subfamily A, Member 5

Alternate Names

ISWI, SNF2H, WCRF135

Gene Symbol

SMARCA5

Additional SMARCA5/SNF2H Products

Product Documents for SMARCA5/SNF2H Antibody (6H8I7)

Certificate of Analysis

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Product Specific Notices for SMARCA5/SNF2H Antibody (6H8I7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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