SMARCA6 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03793

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SMARCA6 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 719-838 of human SMARCA6 (NP_060533.2). VYRLVTANTIDQKIVERAAAKRKLEKLIIHKNHFKGGQSGLNLSKNFLDPKELMELLKSRDYEREIKGSREKVISDKDLELLLDRSDLIDQMNASGPIKEKMGIFKILENSEDSSPECLF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SMARCA6 Antibody - BSA Free

Western Blot: SMARCA6 AntibodyBSA Free [NBP3-03793]

Western Blot: SMARCA6 AntibodyBSA Free [NBP3-03793]

Western Blot: SMARCA6 Antibody [NBP3-03793] - Analysis of extracts of various cell lines, using SMARCA6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
Knockout Validated: SMARCA6 Antibody - BSA Free [NBP3-03793]

Western Blot: SMARCA6 Antibody - BSA Free [NBP3-03793]

Western Blot: SMARCA6 Antibody [NBP3-03793] - Analysis of extracts from normal (control) and HELLS knockout (KO) HeLa cells, using SMARCA6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation: SMARCA6 Antibody - BSA Free [NBP3-03793]

Immunoprecipitation: SMARCA6 Antibody - BSA Free [NBP3-03793]

Immunoprecipitation: SMARCA6 Antibody [NBP3-03793] - Analysis of 200ug extracts of 293T cells using SMARCA6 antibody. Western blot was performed from the immunoprecipitate using this antibody.

Applications for SMARCA6 Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1ug/mL

Immunoprecipitation

0.5μg-4μg antibody for 200ug-400ug extracts of whole ccells

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SMARCA6

HELLS, also known as LSH (lymphoid specific helicase), is a member of the SNF2 superfamily of chromatin helicases. HELLS is ubiquitously expressed and is required for the maintenance of normal DNA methylation patterns important to organism development. HELLS function has also been shown to be important to the regulation of proliferation and the expression of senescence-related genes.

Alternate Names

EC 3.6.1, EC 3.6.4.-, helicase, lymphoid-specific, LSHSWI/SNF2-related, matrix-associated, actin-dependent regulator of chromatin, Nbla10143, PASGFLJ10339, Proliferation-associated SNF2-like protein, SMARCA6lymphoid-specific helicase, subfamily A, member 6, SWI/SNF2-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 6

Gene Symbol

HELLS

Additional SMARCA6 Products

Product Documents for SMARCA6 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SMARCA6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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